CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …

Alzheimer's disease (AD) is an age-related disease pathologically defined by the deposition
of amyloid plaques and neurofibrillary tangles in the brain parenchyma. Single-cell profiling …

Persistent DNA double-strand breaks (DSBs) in neurons are an early pathological hallmark
of neurodegenerative diseases including Alzheimer's disease (AD), with the potential to …

Neuronal activity is crucial for adaptive circuit remodelling but poses an inherent risk to the
stability of the genome across the long lifespan of postmitotic neurons,,,–. Whether neurons …

DNA single-strand breaks (SSBs) are amongst the commonest DNA lesions arising in cells,
with many tens of thousands induced in each cell each day. SSBs arise not only from …

Accurate somatic mutation detection from single-cell DNA sequencing is challenging due to
amplification-related artifacts. To reduce this artifact burden, an improved amplification …

Using CRISPR-Cas9 nicking enzymes, we examined the interaction between the replication
machinery and single-strand breaks, one of the most common forms of endogenous DNA …

Inflammation is a biological response involving immune cells, blood vessels and mediators
induced by endogenous and exogenous stimuli, such as pathogens, damaged cells or …

Neurons harbor high levels of single-strand DNA breaks (SSBs) that are targeted to
neuronal enhancers, but the source of this endogenous damage remains unclear. Using two …

Eating disorders (anorexia nervosa, bulimia nervosa and binge-eating disorder) are a
heterogeneous class of complex illnesses marked by weight and appetite dysregulation …