Quorum quenching enzymes and their effects on virulence, biofilm, and microbiomes: a review of recent advances

R Sikdar, M Elias - Expert review of anti-infective therapy, 2020 - Taylor & Francis
Introduction Numerous bacterial behaviors are regulated by a cell-density dependent
mechanism known as Quorum Sensing (QS). QS relies on communication between bacterial …

Biotechnological applications of quorum quenching enzymes

J Bzdrenga, D Daude, B Remy, P Jacquet… - Chemico-biological …, 2017 - Elsevier
Numerous bacteria use quorum sensing (QS) to synchronize their behavior and monitor
their population density. They use signaling molecules known as autoinducers (AI's) that are …

Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control

R Billot, L Plener, P Jacquet, M Elias… - Journal of Biological …, 2020 - ASBMB
Enzymes able to degrade or modify acyl-homoserine lactones (AHLs) have drawn
considerable interest for their ability to interfere with the bacterial communication process …

Current and emerging strategies for organophosphate decontamination: special focus on hyperstable enzymes

P Jacquet, D Daudé, J Bzdrenga, P Masson… - … Science and Pollution …, 2016 - Springer
Organophosphorus chemicals are highly toxic molecules mainly used as pesticides. Some
of them are banned warfare nerve agents. These compounds are covalent inhibitors of …

Catalytic Redundancies and Conformational Plasticity Drives Selectivity and Promiscuity in Quorum Quenching Lactonases

M Corbella, J Bravo, AO Demkiv, AR Calixto… - JACS Au, 2024 - ACS Publications
Several enzymes from the metallo-β-lactamase-like family of lactonases (MLLs) degrade N-
acyl L-homoserine lactones (AHLs). They play a role in a microbial communication system …

Structural and biochemical characterization of AaL, a quorum quenching lactonase with unusual kinetic properties

C Bergonzi, M Schwab, T Naik, D Daudé… - Scientific reports, 2018 - nature.com
Quorum quenching lactonases are enzymes that are capable of disrupting bacterial
signaling based on acyl homoserine lactones (AHL) via their enzymatic degradation. In …

Rational engineering of a native hyperthermostable lactonase into a broad spectrum phosphotriesterase

P Jacquet, J Hiblot, D Daudé, C Bergonzi, G Gotthard… - Scientific reports, 2017 - nature.com
The redesign of enzyme active sites to alter their function or specificity is a difficult yet
appealing challenge. Here we used a structure-based design approach to engineer the …

Signal disruption leads to changes in bacterial community population

M Schwab, C Bergonzi, J Sakkos, C Staley… - Frontiers in …, 2019 - frontiersin.org
The disruption of bacterial signaling (quorum quenching) has been proven to be an
innovative approach to influence the behavior of bacteria. In particular, lactonase enzymes …

Crystal structure of VmoLac, a tentative quorum quenching lactonase from the extremophilic crenarchaeon Vulcanisaeta moutnovskia

J Hiblot, J Bzdrenga, C Champion, E Chabriere… - Scientific reports, 2015 - nature.com
A new representative of the Phosphotriesterase-Like Lactonases (PLLs) family from the
hyperthermophilic crenarchaeon Vulcanisaeta moutnovskia has been characterized and …

Effects of signal disruption depends on the substrate preference of the lactonase

K Mahan, R Martinmaki, I Larus, R Sikdar… - Frontiers in …, 2020 - frontiersin.org
Many bacteria produce and use extracellular signaling molecules such as acyl homoserine
lactones (AHLs) to communicate and coordinate behavior in a cell-density dependent …