The accurate and complete replication of genomic DNA is essential for all life. In eukaryotic
cells, the assembly of the multi-enzyme replisomes that perform replication is divided into …

The budding yeast Saccharomyces cerevisiae is a powerful model organism for studying
fundamental aspects of eukaryotic cell biology. This Primer article presents a brief historical …

Pangenomes provide access to an accurate representation of the genetic diversity of
species, both in terms of sequence polymorphisms and structural variants (SVs). Here we …

The origin recognition complex (ORC) is essential for initiation of eukaryotic chromosome
replication as it loads the replicative helicase—the minichromosome maintenance (MCM) …

The internal environment of growing cells is variable and dynamic, making it difficult to
introduce reliable parts, such as promoters, for genetic engineering. Here, we applied …

Initiation of eukaryotic DNA replication requires phosphorylation of the MCM complex by
Dbf4-dependent kinase (DDK), composed of Cdc7 kinase and its activator, Dbf4. We report …

Origin recognition complex (ORC)-dependent loading of the replicative helicase MCM2-7
onto replication origins in G1-phase forms the basis of replication fork establishment in S …

Current temporal studies of DNA replication are either low-resolution or require complex cell
synchronisation and/or sorting procedures. Here we introduce Nanotiming, a single …

Most current evidence indicates that DNA polymerases ε and δ, respectively, perform the
bulk of leading and lagging strand replication of the eukaryotic nuclear genome. Given that …

Three eukaryotic DNA polymerases are essential for genome replication. Polymerase (Pol)
α–primase initiates each synthesis event and is rapidly replaced by processive DNA …