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Technologies and standardization in research on extracellular vesicles
Extracellular vesicles (EVs) are phospholipid bilayer membrane-enclosed structures
containing RNAs, proteins, lipids, metabolites, and other molecules, secreted by various …
containing RNAs, proteins, lipids, metabolites, and other molecules, secreted by various …
Isolation of extracellular vesicles: general methodologies and latest trends
MY Konoshenko, EA Lekchnov… - BioMed research …, 2018 - Wiley Online Library
Background. Extracellular vesicles (EVs) play an essential role in the communication
between cells and transport of diagnostically significant molecules. A wide diversity of …
between cells and transport of diagnostically significant molecules. A wide diversity of …
A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs
The use of microRNAs as clinical cancer biomarkers is hindered by the absence of accurate,
fast and inexpensive assays for their detection in biofluids. Here we report a one-step and …
fast and inexpensive assays for their detection in biofluids. Here we report a one-step and …
A comparison of methods for the isolation and separation of extracellular vesicles from protein and lipid particles in human serum
K Brennan, K Martin, SP FitzGerald, J O'sullivan… - Scientific reports, 2020 - nature.com
Extracellular vesicles (EVs) are nano-sized vesicles containing nucleic acid and protein
cargo that are released from a multitude of cell types and have gained significant interest as …
cargo that are released from a multitude of cell types and have gained significant interest as …
Principles and problems of exosome isolation from biological fluids
EI Yakubovich, AG Polischouk… - … ), Supplement Series A …, 2022 - Springer
Exosomes, the subclass of small membrane extracellular vesicles, have great diagnostic
and therapeutic potential, but the lack of standardized methods for their efficient isolation …
and therapeutic potential, but the lack of standardized methods for their efficient isolation …
Methodological guidelines to study extracellular vesicles
Owing to the relationship between extracellular vesicles (EVs) and physiological and
pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes …
pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes …
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA–an ISEV position paper
The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has
been demonstrated in a multitude of different in vitro cell systems and in a variety of body …
been demonstrated in a multitude of different in vitro cell systems and in a variety of body …
Technical challenges of working with extracellular vesicles
Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with
potential applications in diagnosis, prognosis and therapeutic guidance in most pathological …
potential applications in diagnosis, prognosis and therapeutic guidance in most pathological …
A review on comparative studies addressing exosome isolation methods from body fluids
Exosomes emerged as valuable sources of disease biomarkers and new therapeutic tools.
However, extracellular vesicles isolation with exosome-like characteristics from certain …
However, extracellular vesicles isolation with exosome-like characteristics from certain …
Quality and efficiency assessment of six extracellular vesicle isolation methods by nano-flow cytometry
Y Tian, M Gong, Y Hu, H Liu, W Zhang… - Journal of …, 2020 - Taylor & Francis
Extracellular vesicles (EVs) have sparked tremendous interest owing to their prominent
potential in diagnostics and therapeutics. Isolation of EVs from complex biological fluids with …
potential in diagnostics and therapeutics. Isolation of EVs from complex biological fluids with …