Technologies and standardization in research on extracellular vesicles

S Gandham, X Su, J Wood, AL Nocera, SC Alli… - Trends in …, 2020 - cell.com
Extracellular vesicles (EVs) are phospholipid bilayer membrane-enclosed structures
containing RNAs, proteins, lipids, metabolites, and other molecules, secreted by various …

Isolation of extracellular vesicles: general methodologies and latest trends

MY Konoshenko, EA Lekchnov… - BioMed research …, 2018 - Wiley Online Library
Background. Extracellular vesicles (EVs) play an essential role in the communication
between cells and transport of diagnostically significant molecules. A wide diversity of …

A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs

H Yan, Y Wen, Z Tian, N Hart, S Han… - Nature biomedical …, 2023 - nature.com
The use of microRNAs as clinical cancer biomarkers is hindered by the absence of accurate,
fast and inexpensive assays for their detection in biofluids. Here we report a one-step and …

A comparison of methods for the isolation and separation of extracellular vesicles from protein and lipid particles in human serum

K Brennan, K Martin, SP FitzGerald, J O'sullivan… - Scientific reports, 2020 - nature.com
Extracellular vesicles (EVs) are nano-sized vesicles containing nucleic acid and protein
cargo that are released from a multitude of cell types and have gained significant interest as …

Principles and problems of exosome isolation from biological fluids

EI Yakubovich, AG Polischouk… - … ), Supplement Series A …, 2022 - Springer
Exosomes, the subclass of small membrane extracellular vesicles, have great diagnostic
and therapeutic potential, but the lack of standardized methods for their efficient isolation …

Methodological guidelines to study extracellular vesicles

FAW Coumans, AR Brisson, EI Buzas… - Circulation …, 2017 - ahajournals.org
Owing to the relationship between extracellular vesicles (EVs) and physiological and
pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes …

Obstacles and opportunities in the functional analysis of extracellular vesicle RNA–an ISEV position paper

B Mateescu, EJK Kowal, BWM Van Balkom… - Journal of …, 2017 - Taylor & Francis
The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has
been demonstrated in a multitude of different in vitro cell systems and in a variety of body …

Technical challenges of working with extracellular vesicles

MI Ramirez, MG Amorim, C Gadelha, I Milic, JA Welsh… - Nanoscale, 2018 - pubs.rsc.org
Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with
potential applications in diagnosis, prognosis and therapeutic guidance in most pathological …

A review on comparative studies addressing exosome isolation methods from body fluids

TS Martins, M Vaz, AG Henriques - Analytical and Bioanalytical Chemistry, 2023 - Springer
Exosomes emerged as valuable sources of disease biomarkers and new therapeutic tools.
However, extracellular vesicles isolation with exosome-like characteristics from certain …

Quality and efficiency assessment of six extracellular vesicle isolation methods by nano-flow cytometry

Y Tian, M Gong, Y Hu, H Liu, W Zhang… - Journal of …, 2020 - Taylor & Francis
Extracellular vesicles (EVs) have sparked tremendous interest owing to their prominent
potential in diagnostics and therapeutics. Isolation of EVs from complex biological fluids with …