Clinical metagenomics

CY Chiu, SA Miller - Nature Reviews Genetics, 2019 - nature.com
Clinical metagenomic next-generation sequencing (mNGS), the comprehensive analysis of
microbial and host genetic material (DNA and RNA) in samples from patients, is rapidly …

Shotgun metagenomics, from sampling to analysis

C Quince, AW Walker, JT Simpson, NJ Loman… - Nature …, 2017 - nature.com
Diverse microbial communities of bacteria, archaea, viruses and single-celled eukaryotes
have crucial roles in the environment and in human health. However, microbes are …

Binning metagenomic contigs by coverage and composition

J Alneberg, BS Bjarnason, I De Bruijn, M Schirmer… - Nature …, 2014 - nature.com
Shotgun sequencing enables the reconstruction of genomes from complex microbial
communities, but because assembly does not reconstruct entire genomes, it is necessary to …

Recent advances in understanding enteric pathogenic Escherichia coli

MA Croxen, RJ Law, R Scholz… - Clinical microbiology …, 2013 - Am Soc Microbiol
Although Escherichia coli can be an innocuous resident of the gastrointestinal tract, it also
has the pathogenic capacity to cause significant diarrheal and extraintestinal diseases …

Microbial strain-level population structure and genetic diversity from metagenomes

DT Truong, A Tett, E Pasolli, C Huttenhower… - Genome …, 2017 - genome.cshlp.org
Among the human health conditions linked to microbial communities, phenotypes are often
associated with only a subset of strains within causal microbial groups. Although it has been …

Benchmarking metagenomics tools for taxonomic classification

HY Simon, KJ Siddle, DJ Park, PC Sabeti - Cell, 2019 - cell.com
Metagenomic sequencing is revolutionizing the detection and characterization of microbial
species, and a wide variety of software tools are available to perform taxonomic …

The Prevotella copri complex comprises four distinct clades underrepresented in westernized populations

A Tett, KD Huang, F Asnicar, H Fehlner-Peach… - Cell host & …, 2019 - cell.com
Prevotella copri is a common human gut microbe that has been both positively and
negatively associated with host health. In a cross-continent meta-analysis exploiting> 6,500 …

Exploring the human microbiome: the potential future role of next-generation sequencing in disease diagnosis and treatment

MA Malla, A Dubey, A Kumar, S Yadav… - Frontiers in …, 2019 - frontiersin.org
The interaction between the human microbiome and immune system has an effect on
several human metabolic functions and impacts our well-being. Additionally, the interaction …

Hybrid metagenomic assembly enables high-resolution analysis of resistance determinants and mobile elements in human microbiomes

D Bertrand, J Shaw, M Kalathiyappan, AHQ Ng… - Nature …, 2019 - nature.com
Abstract Characterization of microbiomes has been enabled by high-throughput
metagenomic sequencing. However, existing methods are not designed to combine reads …

The use of next generation sequencing for improving food safety: Translation into practice

B Jagadeesan, P Gerner-Smidt, MW Allard, S Leuillet… - Food …, 2019 - Elsevier
Abstract Next Generation Sequencing (NGS) combined with powerful bioinformatic
approaches are revolutionising food microbiology. Whole genome sequencing (WGS) of …