A practical guide to amplicon and metagenomic analysis of microbiome data

YX Liu, Y Qin, T Chen, M Lu, X Qian, X Guo… - Protein & cell, 2021 - academic.oup.com
Advances in high-throughput sequencing (HTS) have fostered rapid developments in the
field of microbiome research, and massive microbiome datasets are now being generated …

Minimum information about an uncultivated virus genome (MIUViG)

S Roux, EM Adriaenssens, BE Dutilh, EV Koonin… - Nature …, 2019 - nature.com
We present an extension of the Minimum Information about any (x) Sequence (MIxS)
standard for reporting sequences of uncultivated virus genomes. Minimum Information about …

GTDB-Tk: a toolkit to classify genomes with the Genome Taxonomy Database

PA Chaumeil, AJ Mussig, P Hugenholtz, DH Parks - 2020 - academic.oup.com
Abstract Summary The Genome Taxonomy Database Toolkit (GTDB-Tk) provides objective
taxonomic assignments for bacterial and archaeal genomes based on the GTDB. GTDB-Tk …

CheckM2: a rapid, scalable and accurate tool for assessing microbial genome quality using machine learning

A Chklovski, DH Parks, BJ Woodcroft, GW Tyson - Nature Methods, 2023 - nature.com
Advances in sequencing technologies and bioinformatics tools have dramatically increased
the recovery rate of microbial genomes from metagenomic data. Assessing the quality of …

A unified catalog of 204,938 reference genomes from the human gut microbiome

A Almeida, S Nayfach, M Boland, F Strozzi… - Nature …, 2021 - nature.com
Comprehensive, high-quality reference genomes are required for functional characterization
and taxonomic assignment of the human gut microbiota. We present the Unified Human …

VirSorter2: a multi-classifier, expert-guided approach to detect diverse DNA and RNA viruses

J Guo, B Bolduc, AA Zayed, A Varsani… - Microbiome, 2021 - Springer
Background Viruses are a significant player in many biosphere and human ecosystems, but
most signals remain “hidden” in metagenomic/metatranscriptomic sequence datasets due to …

MetaBAT 2: an adaptive binning algorithm for robust and efficient genome reconstruction from metagenome assemblies

DD Kang, F Li, E Kirton, A Thomas, R Egan, H An… - PeerJ, 2019 - peerj.com
We previously reported on MetaBAT, an automated metagenome binning software tool to
reconstruct single genomes from microbial communities for subsequent analyses of …

A genomic catalog of Earth's microbiomes

S Nayfach, S Roux, R Seshadri, D Udwary… - Nature …, 2021 - nature.com
The reconstruction of bacterial and archaeal genomes from shotgun metagenomes has
enabled insights into the ecology and evolution of environmental and host-associated …

A complete domain-to-species taxonomy for Bacteria and Archaea

DH Parks, M Chuvochina, PA Chaumeil, C Rinke… - Nature …, 2020 - nature.com
Abstract The Genome Taxonomy Database is a phylogenetically consistent, genome-based
taxonomy that provides rank-normalized classifications for~ 150,000 bacterial and archaeal …

Oxford Nanopore R10. 4 long-read sequencing enables the generation of near-finished bacterial genomes from pure cultures and metagenomes without short-read or …

M Sereika, RH Kirkegaard, SM Karst, TY Michaelsen… - Nature …, 2022 - nature.com
Abstract Long-read Oxford Nanopore sequencing has democratized microbial genome
sequencing and enables the recovery of highly contiguous microbial genomes from isolates …