High-throughput screening by droplet microfluidics: Perspective into key challenges and future prospects

EM Payne, DA Holland-Moritz, S Sun, RT Kennedy - Lab on a Chip, 2020 - pubs.rsc.org
In two decades of development, impressive strides have been made for automating basic
laboratory operations in droplet-based microfluidics, allowing the emergence of a new form …

Droplet microfluidics for microbiology: techniques, applications and challenges

TS Kaminski, O Scheler, P Garstecki - Lab on a Chip, 2016 - pubs.rsc.org
Droplet microfluidics has rapidly emerged as one of the key technologies opening up new
experimental possibilities in microbiology. The ability to generate, manipulate and monitor …

Mass activated droplet sorting (mads) enables high‐throughput screening of enzymatic reactions at nanoliter scale

DA Holland‐Moritz, MK Wismer… - Angewandte Chemie …, 2020 - Wiley Online Library
Microfluidic droplet sorting enables the high‐throughput screening and selection of water‐in‐
oil microreactors at speeds and volumes unparalleled by traditional well‐plate approaches …

Controlling molecular transport in minimal emulsions

P Gruner, B Riechers, B Semin, J Lim… - Nature …, 2016 - nature.com
Emulsions are metastable dispersions in which molecular transport is a major mechanism
driving the system towards its state of minimal energy. Determining the underlying …

Microfluidics for antibiotic susceptibility testing

W Postek, N Pacocha, P Garstecki - Lab on a Chip, 2022 - pubs.rsc.org
The rise of antibiotic resistance is a threat to global health. Rapid and comprehensive
analysis of infectious strains is critical to reducing the global use of antibiotics, as informed …

The future of antibiotics begins with discovering new combinations

M Zhu, MW Tse, J Weller, J Chen… - Annals of the New York …, 2021 - Wiley Online Library
Antibiotic resistance is a worldwide and growing clinical problem. With limited drug
development in the antibacterial space, combination therapy has emerged as a promising …

In-depth analysis of biocatalysts by microfluidics: An emerging source of data for machine learning

M Vasina, D Kovar, J Damborsky, Y Ding, T Yang… - Biotechnology …, 2023 - Elsevier
Nowadays, the vastly increasing demand for novel biotechnological products is supported
by the continuous development of biocatalytic applications that provide sustainable green …

Microdroplet-assisted screening of biomolecule production for metabolic engineering applications

EK Bowman, HS Alper - Trends in biotechnology, 2020 - cell.com
Success in synthetic biology and metabolic engineering is quickly becoming 'test'limited
within the design–build–test cycle. Commonly used methods for high-throughput screening …

Simple and precise counting of viable bacteria by resazurin-amplified picoarray detection

K Hsieh, HC Zec, L Chen, AM Kaushik… - Analytical …, 2018 - ACS Publications
Simple, fast, and precise counting of viable bacteria is fundamental to a variety of
microbiological applications such as food quality monitoring and clinical diagnosis. To this …

Fluorescence-activated droplet sorting of lipolytic microorganisms using a compact optical system

Y Qiao, X Zhao, J Zhu, R Tu, L Dong, L Wang, Z Dong… - Lab on a Chip, 2018 - pubs.rsc.org
Lipases are ubiquitous enzymes of great physiological significance that have been used
extensively in multiple industries. Environmental microorganisms are a major source for the …