Genome editing has been a transformative force in the life sciences and human medicine,
offering unprecedented opportunities to dissect complex biological processes and treat the …

The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …

Programmable genome integration of large, diverse DNA cargo without DNA repair of
exposed DNA double-strand breaks remains an unsolved challenge in genome editing. We …

Conventional genome engineering with CRISPR–Cas9 creates double-strand breaks
(DSBs) that lead to undesirable byproducts and reduce product purity. Here we report an …

Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

Existing technologies for site-specific integration of kilobase-sized DNA sequences in
bacteria are limited by low efficiency, a reliance on recombination, the need for multiple …

The population dynamics of the human microbiome have been associated with inflammatory
bowel disease, cancer, obesity, autoimmune diseases, and many other human disease …

The past decade has witnessed a rapid evolution in identifying more versatile clustered
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …

Advances in synthetic biology and the clinical application of bacteriotherapy enable the use
of genetically engineered bacteria (GEB) to combat various diseases. GEB act as a small …

CRISPR–Cas (clustered regularly interspaced short palindromic repeats–CRISPR-
associated nuclease) defense systems have been naturally coopted for guide RNA–directed …