The Electrospray Ionization (ESI) is a soft ionization technique extensively used for
production of gas phase ions (without fragmentation) of thermally labile large …

The ideas that proteins might possess specific functions without being uniquely folded into
rigid 3D-structures and that these floppy polypeptides might constitute a noticeable part of …

Electrospray ionization (ESI) generates intact gas-phase ions from analytes in solution for
mass spectrometric investigations. ESI can proceed via different mechanisms. Low …

Traditionally, mass spectrometry has been a powerful analytical method enabling the
structural analysis of small molecules, and later on peptides and proteins. With the advent of …

Collapse to compact states in the gas phase, with smaller collision cross sections than
calculated for their native-like structure, has been reported previously for some protein …

Monoclonal antibodies (mAbs) are powerful therapeutics, and their characterization has
drawn considerable attention and urgency. Unlike small-molecule drugs (150–600 Da) that …

Fast photochemical oxidation of proteins (FPOP) is a chemical footprinting method whereby
exposed amino-acid residues are covalently labeled by oxidation with hydroxyl radicals …

The extent to which proteins in the gas phase retain their condensed-phase structure is a
hotly debated issue. Closely related to this is the degree to which the observed charge state …

Understanding the charging mechanism of electrospray ionization is central to overcoming
shortcomings such as ion suppression or limited dynamic range, and explaining …

Electrospray ionization (ESI) of native proteins results in a narrow distribution of low
protonation states. ESI for these folded species proceeds via the charged residue …