Border control: regulating LPS biogenesis

RL Guest, ST Rutherford, TJ Silhavy - Trends in Microbiology, 2021 - cell.com
The outer membrane (OM) is a defining feature of Gram-negative bacteria that serves as a
permeability barrier and provides rigidity to the cell. Critical to OM function is establishing …

Advances in therapeutic bacterial antisense biotechnology

JP Hegarty, DB Stewart - Applied microbiology and biotechnology, 2018 - Springer
Antisense therapeutics are a biotechnological form of antibiotic therapy using chemical
analogues of short single-stranded nucleic acid sequences modified to form stable …

Structure of the essential inner membrane lipopolysaccharide–PbgA complex

T Clairfeuille, KR Buchholz, Q Li, E Verschueren, P Liu… - Nature, 2020 - nature.com
Lipopolysaccharide (LPS) resides in the outer membrane of Gram-negative bacteria where it
is responsible for barrier function,. LPS can cause death as a result of septic shock, and its …

Global landscape of cell envelope protein complexes in Escherichia coli

M Babu, C Bundalovic-Torma, C Calmettes… - Nature …, 2018 - nature.com
Bacterial cell envelope protein (CEP) complexes mediate a range of processes, including
membrane assembly, antibiotic resistance and metabolic coordination. However, only …

YejM modulates activity of the YciM/FtsH protease complex to prevent lethal accumulation of lipopolysaccharide

RL Guest, D Samé Guerra, M Wissler, J Grimm… - MBio, 2020 - Am Soc Microbiol
Lipopolysaccharide (LPS) is an essential glycolipid present in the outer membrane (OM) of
many Gram-negative bacteria. Balanced biosynthesis of LPS is critical for cell viability; too …

An essential membrane protein modulates the proteolysis of LpxC to control lipopolysaccharide synthesis in Escherichia coli

EM Fivenson, TG Bernhardt - MBio, 2020 - Am Soc Microbiol
Gram-negative bacteria are surrounded by a complex cell envelope that includes two
membranes. The outer membrane prevents many drugs from entering these cells and is thus …

Targeting bacterial cardiolipin enriched microdomains: an antimicrobial strategy used by amphiphilic aminoglycoside antibiotics

M El Khoury, J Swain, G Sautrey, L Zimmermann… - Scientific reports, 2017 - nature.com
Some bacterial proteins involved in cell division and oxidative phosphorylation are tightly
bound to cardiolipin. Cardiolipin is a non-bilayer anionic phospholipid found in bacterial …

Structural and functional insights into the lipopolysaccharide ABC transporter LptB2FG

H Dong, Z Zhang, X Tang, NG Paterson… - Nature …, 2017 - nature.com
The cell surface of most Gram-negative bacteria contains lipopolysaccharide that is
essential for their viability and drug resistance. A 134-kDa protein complex LptB2FG is …

YejM controls LpxC levels by regulating protease activity of the FtsH/YciM complex of Escherichia coli

D Nguyen, K Kelly, N Qiu, R Misra - Journal of bacteriology, 2020 - Am Soc Microbiol
LpxC is a deacetylase that catalyzes the first committed step of lipid A biosynthesis in
Escherichia coli. LpxC competes for a common precursor, R-3-hydroxymyristoyl-UDP …

Regulation of the first committed step in lipopolysaccharide biosynthesis catalyzed by LpxC requires the essential protein LapC (YejM) and HslVU protease

D Biernacka, P Gorzelak, G Klein, S Raina - International Journal of …, 2020 - mdpi.com
We previously showed that lipopolysaccharide (LPS) assembly requires the essential LapB
protein to regulate FtsH-mediated proteolysis of LpxC protein that catalyzes the first …