Access to a wide range of plastic materials has been rationalized by the increased demand
from growing populations and the development of high-throughput production systems …

Synthetic cells are engineered vesicles that can mimic one or more salient features of life.
These features include directed localization, sense‐and‐respond behavior, gene …

The design and optimization of biosynthetic pathways for industrially relevant, non-model
organisms is challenging due to transformation idiosyncrasies, reduced numbers of …

Metabolic engineering of microorganisms to produce sustainable chemicals has emerged
as an important part of the global bioeconomy. Unfortunately, efforts to design and engineer …

Biomanufacturing has the potential to reduce demand for petrochemicals and mitigate
climate change. Recent studies have also suggested that some of these products can be net …

Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …

Valinomycin is a nonribosomal peptide that was discovered from Streptomyces in 1955.
Over the past more than six decades, it has received continuous attention due to its special …

Directing CO2 conversion using photoautotrophic microbes offers a promising route to
coupling carbon mitigation with petrochemical replacement. However, solar-based …

Highlights•Cell-free systems enable total biosynthesis of complex natural products.•Cell-free
systems are robust to express long biosynthetic gene clusters (> 10 kb).•Cell-free reactions …

Highlights•Enzymes are core elements of metabolic engineering and biotechnology.•
Enzymatic promiscuity allows development of novel biosynthetic pathways.•Protein …