Antibody-based biotherapeutics have emerged as a successful class of pharmaceuticals
despite significant challenges and risks to their discovery and development. This review …

Antibody drugs should exhibit not only high-binding affinity for their target antigens but also
favorable physicochemical drug-like properties. Such drug-like biophysical properties are …

AlphaFold2 has hallmarked a generational improvement in protein structure prediction. In
particular, advances in antibody structure prediction have provided a highly translatable …

Specifically designed co-solvent mixtures are an efficient way to enhance the kinetics of
enzyme-catalyzed reactions without compromising enzyme stability; among them, several …

Computational methods including machine learning and molecular dynamics simulations
have strong potential to characterize, understand, and ultimately predict the properties of …

Monoclonal antibodies (mAbs) have been immensely successful as biological drugs.
However, the treatment of some diseases requires combinations of antibodies that bind to …

Intrinsic differential scanning fluorimetry (DSF) is essential for analyzing protein thermal
stability. Until now, intrinsic DSF was characterized by medium throughput and high …

Pharmaceutical proteins, compared to small molecular weight drugs, are relatively fragile
molecules, thus necessitating monitoring protein unfolding and aggregation during …

Biopharmaceutical formulations may be compromised by freezing, which has been
attributed to protein conformational changes at a low temperature, and adsorption to ice …

Molecular dynamics simulations were employed to investigate the interaction between Fe
(III) and an iron-binding site composed of THR259, ASP252, and GLU261 on the Fc domain …