Decoding the spermatogenesis program: new insights from transcriptomic analyses

M Rabbani, X Zheng, GL Manske… - Annual review of …, 2022 - annualreviews.org
Spermatogenesis is a complex differentiation process coordinated spatiotemporally across
and along seminiferous tubules. Cellular heterogeneity has made it challenging to obtain …

Spermatogonial stem cells: updates from specification to clinical relevance

S Sharma, J Wistuba, T Pock, S Schlatt… - Human reproduction …, 2019 - academic.oup.com
Human spermatogonia are target for exploration of adult stem cell characteristics and
potential source for the development of therapeutic applications. Almost 50 years ago, Yves …

[HTML][HTML] The mammalian spermatogenesis single-cell transcriptome, from spermatogonial stem cells to spermatids

BP Hermann, K Cheng, A Singh, L Roa-De La Cruz… - Cell reports, 2018 - cell.com
Spermatogenesis is a complex and dynamic cellular differentiation process critical to male
reproduction and sustained by spermatogonial stem cells (SSCs). Although patterns of gene …

Spatiotemporal developmental trajectories in the Arabidopsis root revealed using high-throughput single-cell RNA sequencing

T Denyer, X Ma, S Klesen, E Scacchi, K Nieselt… - Developmental cell, 2019 - cell.com
High-throughput single-cell RNA sequencing (scRNA-seq) is becoming a cornerstone of
developmental research, providing unprecedented power in understanding dynamic …

High-throughput single nucleus total RNA sequencing of formalin-fixed paraffin-embedded tissues by snRandom-seq

Z Xu, T Zhang, H Chen, Y Zhu, Y Lv, S Zhang… - Nature …, 2023 - nature.com
Formalin-fixed paraffin-embedded (FFPE) tissues constitute a vast and valuable patient
material bank for clinical history and follow-up data. It is still challenging to achieve single …

The molecular evolution of spermatogenesis across mammals

F Murat, N Mbengue, SB Winge, T Trefzer, E Leushkin… - Nature, 2023 - nature.com
The testis produces gametes through spermatogenesis and evolves rapidly at both the
morphological and molecular level in mammals,,,,–, probably owing to the evolutionary …

The mouse microbiome is required for sex-specific diurnal rhythms of gene expression and metabolism

BD Weger, C Gobet, J Yeung, E Martin, S Jimenez… - Cell metabolism, 2019 - cell.com
The circadian clock and associated feeding rhythms have a profound impact on metabolism
and the gut microbiome. To what extent microbiota reciprocally affect daily rhythms of …
C Ernst, N Eling, CP Martinez-Jimenez… - Nature …, 2019 - nature.com
Male gametes are generated through a specialised differentiation pathway involving a
series of developmental transitions that are poorly characterised at the molecular level …

[HTML][HTML] Optimizing nervous system-specific gene targeting with Cre driver lines: prevalence of germline recombination and influencing factors

L Luo, MC Ambrozkiewicz, F Benseler, C Chen… - Neuron, 2020 - cell.com
The Cre-loxP system is invaluable for spatial and temporal control of gene knockout,
knockin, and reporter expression in the mouse nervous system. However, we report varying …

Testis single-cell RNA-seq reveals the dynamics of de novo gene transcription and germline mutational bias in Drosophila

E Witt, S Benjamin, N Svetec, L Zhao - Elife, 2019 - elifesciences.org
The testis is a peculiar tissue in many respects. It shows patterns of rapid gene evolution and
provides a hotspot for the origination of genetic novelties such as de novo genes …