This article presents an overview of time-resolved (lifetime) fluorescence techniques used in
biomedical diagnostics. In particular, we review the development of time-resolved …

Visualization of biological processes and pathologic conditions at the cellular and tissue
levels largely relies on the use of fluorescence intensity signals from fluorophores or their …

NAD is a key determinant of cellular energy metabolism. In contrast, its phosphorylated form,
NADP, plays a central role in biosynthetic pathways and antioxidant defence. The reduced …

Molecular, morphological, and physiological heterogeneity is the inherent property of cells
which governs differences in their response to external influence. Tumor cell metabolic …

Fluorescence guided surgery (FGS) is an imaging technique that allows the surgeon to
visualise different structures and types of tissue during a surgical procedure that may not be …

Integrating biological imaging into early stages of the drug discovery process can provide
invaluable readouts of drug activity within complex disease settings, such as cancer …

A fully-integrated single-photon avalanche diode (SPAD) and time-to-digital converter (TDC)
array for high-speed fluorescence lifetime imaging microscopy (FLIM) in standard 130 nm …

A wide-field microscope with epi-fluorescence and selective plane illumination was
combined with a single-photon avalanche diode (SPAD) array camera to enable live-cell …

Fluorescence lifetime imaging (FLIm) is an optical spectroscopic imaging technique capable
of real‐time assessments of tissue properties in clinical settings. Label‐free FLIm is sensitive …

Cellular responses to oxygen tension have been studied extensively. Oxygen tension can
be determined by considering the phosphorescence lifetime of a phosphorescence sensor …