CRISPR-Cas9 system: A new-fangled dawn in gene editing
Till date, only three techniques namely Zinc Finger Nuclease (ZFN), Transcription-Activator
Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic …
Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic …
Disruption of Intracellular Calcium Homeostasis as a Therapeutic Target Against Trypanosoma cruzi
There is no effective cure for Chagas disease, which is caused by infection with the
arthropod-borne parasite, Trypanosoma cruzi. In the search for new drugs to treat Chagas …
arthropod-borne parasite, Trypanosoma cruzi. In the search for new drugs to treat Chagas …
A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids
T Beneke, R Madden, L Makin… - Royal Society …, 2017 - royalsocietypublishing.org
Clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR-associated
gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens …
gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens …
Expanding the toolbox for Trypanosoma cruzi: A parasite line incorporating a bioluminescence-fluorescence dual reporter and streamlined CRISPR/Cas9 functionality for rapid …
Background Infection with Trypanosoma cruzi causes Chagas disease, a major public
health problem throughout Latin America. There is no vaccine and the only drugs have …
health problem throughout Latin America. There is no vaccine and the only drugs have …
Rapid, selection-free, high-efficiency genome editing in protozoan parasites using CRISPR-Cas9 ribonucleoproteins
Trypanosomatids (order Kinetoplastida), including the human pathogens Trypanosoma cruzi
(agent of Chagas disease), Trypanosoma brucei,(African slee** sickness), and …
(agent of Chagas disease), Trypanosoma brucei,(African slee** sickness), and …
IP3 receptor-mediated Ca2+ release from acidocalcisomes regulates mitochondrial bioenergetics and prevents autophagy in Trypanosoma cruzi
In contrast to animal cells, the inositol 1, 4, 5-trisphosphate receptor of Trypanosoma cruzi
(TcIP 3 R) localizes to acidocalcisomes instead of the endoplasmic reticulum. Here, we …
(TcIP 3 R) localizes to acidocalcisomes instead of the endoplasmic reticulum. Here, we …
LeishGEdit: a method for rapid gene knockout and tagging using CRISPR-Cas9
Postgenomic analyses of Leishmania biology benefit from rapid and precise methods for
gene manipulation. Traditional methods of gene knockout or tagging by homologous …
gene manipulation. Traditional methods of gene knockout or tagging by homologous …
CRISPR/Cas9-mediated gene modification and gene knock out in the human-infective parasite Trichomonas vaginalis
BD Janssen, YP Chen, BM Molgora, SE Wang… - Scientific reports, 2018 - nature.com
The sexually-transmitted parasite Trichomonas vaginalis infects~ 1/4 billion people
worldwide. Despite its prevalence and myriad adverse outcomes of infection, the …
worldwide. Despite its prevalence and myriad adverse outcomes of infection, the …