The choice of reference genes that are stably expressed amongst treatment groups is a
crucial step in real-time quantitative PCR gene expression studies. Recent guidelines have …

Use of PCR in the field of molecular diagnostics has increased to the point where it is now
accepted as the standard method for detecting nucleic acids from a number of sample and …

Today, quantitative real-time PCR is the method of choice for rapid and reliable
quantification of mRNA transcription. However, for an exact comparison of mRNA …

Background The elucidation of gene expression patterns leads to a better understanding of
biological processes. Real-time quantitative RT-PCR has become the standard method for …

Background Usually the reference genes used in gene expression analysis have been
chosen for their known or suspected housekee** roles, however the variation observed in …

Background Research using the zebrafish model has experienced a rapid growth in recent
years. Although real-time reverse transcription PCR (QPCR), normalized to an internal …

Background Real-time quantitative PCR (qPCR) is a method for rapid and reliable
quantification of mRNA transcription. Internal standards such as reference genes are used to …

Background Real-time quantitative reverse transcription PCR (RT-qPCR) data needs to be
normalized for its proper interpretation. Housekee** genes are routinely employed for this …

Background One requisite of quantitative reverse transcription PCR (qRT-PCR) is to
normalise the data with an internal reference gene that is invariant regardless of treatment …

The brown planthopper (BPH), Nilaparvata lugens (Hemiptera, Delphacidae), is one of the
most important rice pests. Abundant genetic studies on BPH have been conducted using …