Small RNA sorting: matchmaking for Argonautes

B Czech, GJ Hannon - Nature Reviews Genetics, 2011 - nature.com
Small RNAs directly or indirectly impact nearly every biological process in eukaryotic cells.
To perform their myriad roles, not only must precise small RNA species be generated, but …

Making risc

T Kawamata, Y Tomari - Trends in biochemical sciences, 2010 - cell.com
It is well established that 20-to 30-nt small RNAs, including small interfering RNAs,
microRNAs and Piwi-interacting RNAs, play crucial roles in regulating gene expression and …

Re-evaluation of the roles of DROSHA, Exportin 5, and DICER in microRNA biogenesis

YK Kim, B Kim, VN Kim - Proceedings of the National …, 2016 - National Acad Sciences
Biogenesis of canonical microRNAs (miRNAs) involves multiple steps: nuclear processing of
primary miRNA (pri-miRNA) by DROSHA, nuclear export of precursor miRNA (pre-miRNA) …

A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity

D Cifuentes, H Xue, DW Taylor, H Patnode, Y Mishima… - Science, 2010 - science.org
Dicer is a central enzyme in microRNA (miRNA) processing. We identified a Dicer-
independent miRNA biogenesis pathway that uses Argonaute2 (Ago2) slicer catalytic …

Alternative miRNA biogenesis pathways and the interpretation of core miRNA pathway mutants

JS Yang, EC Lai - Molecular cell, 2011 - cell.com
Since the establishment of a canonical animal microRNA biogenesis pathway driven by the
RNase III enzymes Drosha and Dicer, an unexpected variety of alternative mechanisms that …

miRNA–target chimeras reveal miRNA 3′-end pairing as a major determinant of Argonaute target specificity

MJ Moore, TKH Scheel, JM Luna, CY Park… - Nature …, 2015 - nature.com
Abstract microRNAs (miRNAs) act as sequence-specific guides for Argonaute (AGO)
proteins, which mediate posttranscriptional silencing of target messenger RNAs. Despite …

Conserved vertebrate mir-451 provides a platform for Dicer-independent, Ago2-mediated microRNA biogenesis

JS Yang, T Maurin, N Robine… - Proceedings of the …, 2010 - National Acad Sciences
Canonical animal microRNAs (miRNAs) are generated by sequential cleavage of precursor
substrates by the Drosha and Dicer RNase III enzymes. Several variant pathways exploit …

Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes

MM Temoche-Diaz, MJ Shurtleff, RM Nottingham… - Elife, 2019 - elifesciences.org
Extracellular vesicles (EVs) encompass a variety of vesicles secreted into the extracellular
space. EVs have been implicated in promoting tumor metastasis, but the molecular …

Deep-sequencing of human Argonaute-associated small RNAs provides insight into miRNA sorting and reveals Argonaute association with RNA fragments of diverse …

AM Burroughs, Y Ando, ML de Hoon, Y Tomaru… - RNA biology, 2011 - Taylor & Francis
While several studies have focused on the relationship between individual miRNA loci or
classes of small RNA with human Argonaute (AGO) proteins, a comprehensive, global …

EBV and human microRNAs co‐target oncogenic and apoptotic viral and human genes during latency

KJ Riley, GS Rabinowitz, TA Yario, JM Luna… - The EMBO …, 2012 - embopress.org
Epstein–Barr virus (EBV) controls gene expression to transform human B cells and maintain
viral latency. High‐throughput sequencing and crosslinking immunoprecipitation (HITS …