Synthetic biology for microbial heavy metal biosensors

HJ Kim, H Jeong, SJ Lee - Analytical and bioanalytical chemistry, 2018 - Springer
Using recombinant DNA technology, various whole-cell biosensors have been developed
for detection of environmental pollutants, including heavy metal ions. Whole-cell biosensors …

Heavy metal whole-cell biosensors using eukaryotic microorganisms: an updated critical review

JC Gutiérrez, F Amaro, A Martín-González - Frontiers in microbiology, 2015 - frontiersin.org
This review analyzes the advantages and disadvantages of using eukaryotic
microorganisms to design whole-cell biosensors (WCBs) for monitoring environmental …

Whole‐cell biosensors for detection of heavy metal ions in environmental samples based on metallothionein promoters from Tetrahymena thermophila

F Amaro, AP Turkewitz… - Microbial …, 2011 - Wiley Online Library
Heavy metals are among the most serious pollutants, and thus there is a need to develop
sensitive and rapid biomonitoring methods for heavy metals in the environment. Critical …

[HTML][HTML] Stress and Protists: No life without stress

V Slaveykova, B Sonntag, JC Gutiérrez - European journal of protistology, 2016 - Elsevier
We report a summary of the symposium “Stress and Protists: No life without stress”, which
was held in September 2015 on the VII European Congress of Protistology in partnership …

[HTML][HTML] Arsenate and arsenite differential toxicity in Tetrahymena thermophila

D Rodríguez-Martín, A Murciano, M Herráiz… - Journal of Hazardous …, 2022 - Elsevier
A comparative analysis of toxicities of both arsenic forms (arsenite and arsenate) in the
model eukaryotic microorganism Tetrahymena thermophila (ciliate protozoa) has shown the …

[HTML][HTML] Copper Bioremediation Ability of Ciliate Paramecium multimicronucleatum Isolated from Industrial Wastewater

A Liaqat, I Zahra, SZ Abbas, SM Wabaidur… - Water, 2022 - mdpi.com
The growing problems of environmental damage have been caused by the continuous
outrush of heavy metals from industrial wastewater. To resolve this issue, bioremediation is …

Functional GFP-metallothionein fusion protein from Tetrahymena thermophila: a potential whole-cell biosensor for monitoring heavy metal pollution and a cell model …

F Amaro, AP Turkewitz, A Martín-González… - Biometals, 2014 - Springer
The significance of metal (oid) s as environmental pollutants has made them a priority in
ecotoxicology, with the aim of minimizing exposure to animals or humans. Therefore, it is …

Ciliate metallothioneins: unique microbial eukaryotic heavy-metal-binder molecules

JC Gutiérrez, F Amaro, S Diaz, P De Francisco… - JBIC Journal of …, 2011 - Springer
This article represents an updated review of ciliate metallothioneins (Tetrahymena species)
including a comparative analysis with regard to well-known metallothioneins (MTs) from …

Microbial impact to environmental toxicants Ni (II) and Co (II): Joint toxicity and cellular response in Paramecium

DLG Amaya, A Thiel, M Möller, G Gasparoni… - Chemosphere, 2023 - Elsevier
Abstract Cobalt (Co) and Nickel (Ni) are increasingly found in our environment. We analysed
their combined toxicity and uptake mechanisms in the early food chain by studying bacteria …

From heavy metal‐binders to biosensors: Ciliate metallothioneins discussed

JC Gutierrez, F Amaro, A Martín‐González - BioEssays, 2009 - Wiley Online Library
Metallothioneins (MTs) are ubiquitous proteins with the capacity to bind heavy metal ions
(mainly Cd, Zn or Cu), and they have been found in animals, plants, eukaryotic and …