Super-resolution microscopy demystified
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …
restricts the optical resolution to roughly 250 nm and was previously thought to be …
[HTML][HTML] The self-organizing genome: principles of genome architecture and function
T Misteli - Cell, 2020 - cell.com
Genomes have complex three-dimensional architectures. The recent convergence of
genetic, biochemical, biophysical, and cell biological methods has uncovered several …
genetic, biochemical, biophysical, and cell biological methods has uncovered several …
Dynamics of CTCF-and cohesin-mediated chromatin loo** revealed by live-cell imaging
Animal genomes are folded into loops and topologically associating domains (TADs) by
CTCF and loop-extruding cohesins, but the live dynamics of loop formation and stability …
CTCF and loop-extruding cohesins, but the live dynamics of loop formation and stability …
The spatial organization of transcriptional control
In animals, the sequences for controlling gene expression do not concentrate just at the
transcription start site of genes, but are frequently thousands to millions of base pairs distal …
transcription start site of genes, but are frequently thousands to millions of base pairs distal …
Principles of genome folding into topologically associating domains
Understanding the mechanisms that underlie chromosome folding within cell nuclei is
essential to determine the relationship between genome structure and function. The recent …
essential to determine the relationship between genome structure and function. The recent …
In situ genome sequencing resolves DNA sequence and structure in intact biological samples
INTRODUCTION Genomes are spatially organized across length scales from single base
pairs to whole chromosomes. This organization is thought to regulate gene expression and …
pairs to whole chromosomes. This organization is thought to regulate gene expression and …
Visualizing DNA folding and RNA in embryos at single-cell resolution
The establishment of cell types during development requires precise interactions between
genes and distal regulatory sequences. We have a limited understanding of how these …
genes and distal regulatory sequences. We have a limited understanding of how these …
[HTML][HTML] Extensive heterogeneity and intrinsic variation in spatial genome organization
Several general principles of global 3D genome organization have recently been
established, including non-random positioning of chromosomes and genes in the cell …
established, including non-random positioning of chromosomes and genes in the cell …
Cis-regulatory chromatin loops arise before TADs and gene activation, and are independent of cell fate during early Drosophila development
Acquisition of cell fate is thought to rely on the specific interaction of remote cis-regulatory
modules (CRMs), for example, enhancers and target promoters. However, the precise …
modules (CRMs), for example, enhancers and target promoters. However, the precise …
Molecular basis and biological function of variability in spatial genome organization
EH Finn, T Misteli - Science, 2019 - science.org
BACKGROUND The eukaryotic genome is hierarchically organized in the cell nucleus into
DNA loops, chromatin domains, compartments, and, ultimately, chromosomes. Many of the …
DNA loops, chromatin domains, compartments, and, ultimately, chromosomes. Many of the …