[HTML][HTML] Dynamic nuclear polarization for sensitivity enhancement in modern solid-state NMR

ASL Thankamony, JJ Wittmann, M Kaushik… - Progress in nuclear …, 2017 - Elsevier
The field of dynamic nuclear polarization has undergone tremendous developments and
diversification since its inception more than 6 decades ago. In this review we provide an in …

Solid-state NMR: Methods for biological solids

S Ahlawat, KR Mote, NA Lakomek… - Chemical Reviews, 2022 - ACS Publications
In the last two decades, solid-state nuclear magnetic resonance (ssNMR) spectroscopy has
transformed from a spectroscopic technique investigating small molecules and industrial …

Facing and overcoming sensitivity challenges in biomolecular NMR spectroscopy

JH Ardenkjaer‐Larsen, GS Boebinger… - Angewandte Chemie …, 2015 - Wiley Online Library
In the Spring of 2013, NMR spectroscopists convened at the Weizmann Institute in Israel to
brainstorm on approaches to improve the sensitivity of NMR experiments, particularly when …

Structural insights from lipid-bilayer nanodiscs link α-Synuclein membrane-binding modes to amyloid fibril formation

T Viennet, MM Wördehoff, B Uluca, C Poojari… - Communications …, 2018 - nature.com
The protein α-Synuclein (αS) is linked to Parkinson's disease through its abnormal
aggregation, which is thought to involve cytosolic and membrane-bound forms of αS …

DNP NMR of biomolecular assemblies

K Jaudzems, T Polenova, G Pintacuda… - Journal of structural …, 2019 - Elsevier
Abstract Dynamic Nuclear Polarization (DNP) is an effective approach to alleviate the
inherently low sensitivity of solid-state NMR (ssNMR) under magic angle spinning (MAS) …

Solid-state dynamic nuclear polarization at 9.4 and 18.8 T from 100 K to room temperature

M Lelli, SR Chaudhari, D Gajan… - Journal of the …, 2015 - ACS Publications
Efficient dynamic nuclear polarization (DNP) in solids, which enables very high sensitivity
NMR experiments, is currently limited to temperatures of around 100 K and below. Here we …

Solid-state NMR on bacterial cells: selective cell wall signal enhancement and resolution improvement using dynamic nuclear polarization

H Takahashi, I Ayala, M Bardet… - Journal of the …, 2013 - ACS Publications
Dynamic nuclear polarization (DNP) enhanced solid-state nuclear magnetic resonance
(NMR) has recently emerged as a powerful technique for the study of material surfaces. In …

Efficient assignment and NMR analysis of an intact virus using sequential side-chain correlations and DNP sensitization

IV Sergeyev, B Itin, R Rogawski… - Proceedings of the …, 2017 - National Acad Sciences
An experimental strategy has been developed to increase the efficiency of dynamic nuclear
polarization (DNP) in solid-state NMR studies. The method makes assignments simpler …

NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field

EJ Koers, EAW van Der Cruijsen, M Rosay… - Journal of biomolecular …, 2014 - Springer
Dynamic nuclear polarization (DNP) has become a powerful method to enhance
spectroscopic sensitivity in the context of magnetic resonance imaging and nuclear …

Matrix-free dynamic nuclear polarization enables solid-state NMR 13 C–13 C correlation spectroscopy of proteins at natural isotopic abundance

H Takahashi, S Hediger, G De Paëpe - Chemical Communications, 2013 - pubs.rsc.org
We introduce a general approach for dynamic nuclear polarization (DNP) enhanced solid-
state NMR that overcomes the current problems in DNP experiments caused by the use of …