In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Fluorescent tools have revolutionized our ability to probe biological dynamics, particularly at
the cellular level. Fluorescent sensors have been developed on several platforms, utilizing …

PARP1, an established anti-cancer target that regulates many cellular pathways, including
DNA repair signaling, has been intensely studied for decades as a poly (ADP-ribosyl) …

The removal of misfolded, ubiquitinated proteins is an essential part of the protein quality
control. The ubiquitin‐proteasome system (UPS) and autophagy are two interconnected …

High-content screening (HCS), which combines automated fluorescence microscopy with
quantitative image analysis, allows the acquisition of unbiased multiparametric data at the …

Single-molecule biophysics has transformed our understanding of biology, but also of the
physics of life. More exotic than simple soft matter, biomatter lives far from thermal …

Protein kinase B/Akt regulates cellular metabolism, survival, and proliferation in response to
hormones and growth factors. Hyperactivation of Akt is frequently observed in cancer, while …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

Genome dynamics are intimately linked to the regulation of gene expression, the most
fundamental mechanism in biology, yet we still do not know whether the very process of …

A fundamental structural and functional change of the human genome is the compaction of
replicated interphase chromatin into rod-shaped mitotic chromosomes. This process of …