Fluorescence correlation spectroscopy (FCS) is a powerful technique to measure
concentrations, mobilities, and interactions of fluorescent biomolecules. It can be applied to …

Investigation of lipid lateral mobility in biological membranes and their artificial models
provides information on membrane dynamics and structure; methods based on optical …

Single-plane illumination (SPIM) or total internal reflection fluorescence (TIRF) microscopes
can be combined with fast and single-molecule-sensitive cameras to allow spatially resolved …

Single-molecule techniques are powerful tools to investigate the structure and dynamics of
macromolecular complexes; however, data quality can suffer because of weak specific …

Fluorescence correlation spectroscopy (FCS) is a single molecule sensitive tool for the
quantitative measurement of biomolecular dynamics and interactions. Improvements in …

Super-resolution microscopy and single molecule fluorescence spectroscopy require
mutually exclusive experimental strategies optimizing either temporal or spatial resolution …

The plasma membrane is a complex fluid where lipids and proteins undergo diffusive motion
critical to biochemical reactions. Through quantitative imaging analyses such as single …

Diffusion processes and local dynamic equilibria inside cells lead to nonuniform spatial
distributions of molecules, which are essential for processes such as nuclear organization …

An Introduction to Fluorescence Correlation Spectroscopy represents a comprehensive
introduction to fluorescence correlation spectroscopy (FCS), a biophysical experimental …

The life sciences require new highly sensitive imaging tools, which allow the quantitative
measurement of molecular parameters within a physiological three-dimensional (3D) …