In recent years, powerful genetic code reprogramming methods have emerged that allow
new functional components to be embedded into proteins as noncanonical amino acid …

The development of new orthogonal aminoacyl-tRNA synthetase/tRNA pairs has led to the
addition of approximately 70 unnatural amino acids (UAAs) to the genetic codes of …

The introduction of chemically unique groups into proteins by means of non-natural amino
acids has numerous applications in protein engineering and functional studies. One method …

We describe a general and rapid route for the addition of unnatural amino acids to the
genetic code of Saccharomyces cerevisiae. Five amino acids have been incorporated into …

Recently, a general method was developed that makes it possible to genetically encode
unnatural amino acids with diverse physical, chemical, or biological properties in …

Misfolded proteins are associated with several pathological conditions including
neurodegeneration. Although some of these abnormally folded proteins result from …

Although chemists can synthesize virtually any small organic molecule, our ability to
rationally manipulate the structures of proteins is quite limited, despite their involvement in …

Protein-protein interactions are the key to organizing cellular processes in space and time.
The only direct way to identify such interactions in their cellular environment is by photo …

The matrix metalloproteinases (MMP)-2 and-9, also known as the gelatinases have been
long recognized as major contributors to the proteolytic degradation of extracellular matrix …

The genetic code is degenerate, in that 20 amino acids are encoded by 61 triplet codes. In
1966, Francis Crick hypothesized that the cell's limited number of tRNAs decoded the …