Over the past two decades, interest in human microbiome research has increased
exponentially. Regrettably, this increased activity has brought with it a degree of hype and …

Although there is now an extensive understanding of the diversity of microbial life on earth
through culture-independent metagenomic DNA sequence analyses, the isolation and …

The gut microbiota has been recognized as an important factor in the development of
metabolic diseases such as obesity and is considered an endocrine organ involved in the …

Obesity is a major risk factor for develo** gallstone disease (GSD). Previous studies have
shown that obesity is associated with an elevated Firmicutes/Bacteroidetes ratio in the gut …

Technical variation in metagenomic analysis must be minimized to confidently assess the
contributions of microbiota to human health. Here we tested 21 representative DNA …

Diverse microbial communities of bacteria, archaea, viruses and single-celled eukaryotes
have crucial roles in the environment and in human health. However, microbes are …

The development and continuous improvement of high-throughput sequencing platforms
have stimulated interest in the study of complex microbial communities. Currently, the most …

Background It has been reported that the composition of human gut microbiota changes with
age; however, few studies have used molecular techniques to investigate the long-term …

One of the major methods to identify microbial community composition, to unravel microbial
population dynamics, and to explore microbial diversity in environmental samples is high …

In order for human microbiome studies to translate into actionable outcomes for health, meta-
analysis of reproducible data from population-scale cohorts is needed. Achieving sufficient …