[HTML][HTML] Nanopore sequencing technology, bioinformatics and applications

Y Wang, Y Zhao, A Bollas, Y Wang, KF Au - Nature biotechnology, 2021 - nature.com
Rapid advances in nanopore technologies for sequencing single long DNA and RNA
molecules have led to substantial improvements in accuracy, read length and throughput …

Genomics in the long-read sequencing era

EL van Dijk, D Naquin, K Gorrichon, Y Jaszczyszyn… - Trends in Genetics, 2023 - cell.com
Long-read sequencing (LRS) technologies have provided extremely powerful tools to
explore genomes. While in the early years these methods suffered technical limitations, they …

Twenty years of plant genome sequencing: achievements and challenges

Y Sun, L Shang, QH Zhu, L Fan, L Guo - Trends in Plant Science, 2022 - cell.com
Publication of the complete genome sequence of Arabidopsis thaliana, the first plant
reference genome, in December 2000 heralded the beginning of the plant genome era …

Time-and memory-efficient genome assembly with Raven

R Vaser, M Šikić - Nature Computational Science, 2021 - nature.com
Whole genome sequencing technologies are unable to invariably read DNA molecules
intact, a shortcoming that assemblers try to resolve by stitching the obtained fragments back …

NextPolish: a fast and efficient genome polishing tool for long-read assembly

J Hu, J Fan, Z Sun, S Liu - Bioinformatics, 2020 - academic.oup.com
Motivation Although long-read sequencing technologies can produce genomes with long
contiguity, they suffer from high error rates. Thus, we developed NextPolish, a tool that …

Fast and accurate long-read assembly with wtdbg2

J Ruan, H Li - Nature methods, 2020 - nature.com
Existing long-read assemblers require thousands of central processing unit hours to
assemble a human genome and are being outpaced by sequencing technologies in terms of …

BRAD V3. 0: an upgraded Brassicaceae database

H Chen, T Wang, X He, X Cai, R Lin… - Nucleic Acids …, 2022 - academic.oup.com
Abstract The Brassicaceae Database (BRAD version 3.0, BRAD V3. 0; http://brassicadb. cn)
has evolved from the former Brassica Database (BRAD V2. 0), and represents an important …

Assembly of allele-aware, chromosomal-scale autopolyploid genomes based on Hi-C data

X Zhang, S Zhang, Q Zhao, R Ming, H Tang - Nature plants, 2019 - nature.com
Construction of chromosome-level assembly is a vital step in achieving the goal of a
'Platinum'genome, but it remains a major challenge to assemble and anchor sequences to …

Large-scale gene expression alterations introduced by structural variation drive morphotype diversification in Brassica oleracea

X Li, Y Wang, C Cai, J Ji, F Han, L Zhang, S Chen… - Nature Genetics, 2024 - nature.com
Brassica oleracea, globally cultivated for its vegetable crops, consists of very diverse
morphotypes, characterized by specialized enlarged organs as harvested products. This …

Tandem repeats lead to sequence assembly errors and impose multi-level challenges for genome and protein databases

OK Tørresen, B Star, P Mier… - Nucleic acids …, 2019 - academic.oup.com
The widespread occurrence of repetitive stretches of DNA in genomes of organisms across
the tree of life imposes fundamental challenges for sequencing, genome assembly, and …