Adeno-associated virus toolkit to target diverse brain cells

RC Challis, S Ravindra Kumar, X Chen… - Annual review of …, 2022 - annualreviews.org
Recombinant adeno-associated viruses (AAVs) are commonly used gene delivery vehicles
for neuroscience research. They have two engineerable features: the capsid (outer protein …

Viral tools for neuroscience

AR Nectow, EJ Nestler - Nature Reviews Neuroscience, 2020 - nature.com
Recombinant viruses are the workhorse of modern neuroscience. Whether one would like to
understand a neuron's morphology, natural activity patterns, molecular composition …

Viral manipulation of functionally distinct interneurons in mice, non-human primates and humans

D Vormstein-Schneider, JD Lin, KA Pelkey… - Nature …, 2020 - nature.com
Recent success in identifying gene-regulatory elements in the context of recombinant adeno-
associated virus vectors has enabled cell-type-restricted gene expression. However, within …

Spatial transcriptomics for profiling the tropism of viral vectors in tissues

MJ Jang, GM Coughlin, CR Jackson, X Chen… - Nature …, 2023 - nature.com
A barrier to advancing engineered adeno-associated viral vectors (AAVs) for precision
access to cell subtypes is a lack of high-throughput, high-resolution assays to characterize in …

[HTML][HTML] Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex

JK Mich, LT Graybuck, EE Hess, JT Mahoney, Y Kojima… - Cell reports, 2021 - cell.com
Viral genetic tools that target specific brain cell types could transform basic neuroscience
and targeted gene therapy. Here, we use comparative open chromatin analysis to identify …

Non-linear dimensionality reduction on extracellular waveforms reveals cell type diversity in premotor cortex

EK Lee, H Balasubramanian, A Tsolias, SU Anakwe… - Elife, 2021 - elifesciences.org
Cortical circuits are thought to contain a large number of cell types that coordinate to
produce behavior. Current in vivo methods rely on clustering of specified features of …

Local feedback inhibition tightly controls rapid formation of hippocampal place fields

SV Rolotti, MS Ahmed, M Szoboszlay, T Geiller… - Neuron, 2022 - cell.com
Hippocampal place cells underlie spatial navigation and memory. Remarkably, CA1
pyramidal neurons can form new place fields within a single trial by undergoing rapid …

Mesoscopic calcium imaging in a head-unrestrained male non-human primate using a lensless microscope

J Wu, Y Chen, A Veeraraghavan, E Seidemann… - Nature …, 2024 - nature.com
Mesoscopic calcium imaging enables studies of cell-type specific neural activity over large
areas. A growing body of literature suggests that neural activity can be different when …

Sexually divergent mortality and partial phenotypic rescue after gene therapy in a mouse model of Dravet syndrome

Y Niibori, SJ Lee, BA Minassian… - Human gene therapy, 2020 - liebertpub.com
Dravet syndrome (DS) is a neurodevelopmental genetic disorder caused by mutations in the
SCN1A gene encoding the α subunit of the NaV1. 1 voltage-gated sodium channel that …

Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy

L Asan, C Falfan-Melgoza, CA Beretta, M Sack… - Scientific Reports, 2021 - nature.com
Magnetic resonance imaging (MRI) of the brain combined with voxel-based morphometry
(VBM) revealed changes in gray matter volume (GMV) in various disorders. However, the …