A great many cell types are necessary for the myriad capabilities of complex, multicellular
organisms. One interesting aspect of this diversity of cell type is that many cells in diploid …

DNA replication must be tightly regulated to ensure that the genome is accurately duplicated
during each cell cycle. When these regulatory mechanisms fail, replicative stress and DNA …

The cell-cycle transition from G 1 to S phase has been difficult to visualize. We have
harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop …

DNA replication is central to cell proliferation. Studies in the past six decades since the
proposal of a semiconservative mode of DNA replication have confirmed the high degree of …

Cul4 E3 ubiquitin ligases contain the cullin 4 scaffold and the triple β propeller Ddb1 adaptor
protein, but few substrate receptors have been identified. Here, we identify 18 Ddb1-and …

Proliferating cell nuclear antigen (PCNA) has a central role in promoting faithful DNA
replication, providing a molecular platform that facilitates the myriad protein–protein and …

The evolutionarily conserved cullin family proteins can assemble as many as 400 distinct E3
ubiquitin ligase complexes that regulate diverse cellular pathways. CUL4, one of three …

Cellular DNA replication is initiated through the action of multiprotein complexes that
recognize replication start sites in the chromosome (termed origins) and facilitate duplex …

The DNA polymerase delta processivity factor Proliferating Cell Nuclear Antigen (PCNA)
promotes the DNA damage-induced degradation of the replication initiation factor Cdt1 via …

In eukaryotic cells, prereplication complexes (pre-RCs) are assembled on chromatin in the
G1 phase, rendering origins of DNA replication competent to initiate DNA synthesis. When …