The frequent discrepancy between direct microscopic counts and numbers of culturable
bacteria from environmental samples is just one of several indications that we currently …

After nearly 10 years of PCR-based analysis of prokaryotic small-subunit ribosomal RNAs
for ecological studies it seems necessary to summarize reported pitfalls of this approach …

Abstract Ribosomal Database Project (RDP; http://rdp. cme. msu. edu/) provides the
research community with aligned and annotated rRNA gene sequence data, along with tools …

ABSTRACT rRNA-based studies, which have become the most common method for
assessing microbial communities, rely upon faithful amplification of the corresponding genes …

A guide to machine learning approaches and their application to the analysis of biological
data. An unprecedented wealth of data is being generated by genome sequencing projects …

Sequence heterogeneities in 16S rRNA genes from individual strains of Paenibacillus
polymyxa were detected by sequence-dependent separation of PCR products by …

The current perception of evolutionary relationships and the natural diversity of ammonia-
oxidizing bacteria (AOB) is mainly based on comparative sequence analyses of their genes …

Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rDNA fragments was
used to explore the genetic diversity of hydrothermal vent microbial communities, specifically …

A 16S rRNA‐based polymerase chain reaction (PCR) detection method was used to
determine the prevalence of Actinobacillus actinomycetemcomitans, Bacteroides forsythus …

A dissimilatory metal-and sulfur-reducing microorganism was isolated from surface
sediments of a hydrocarbon-contaminated ditch in Norman, Okla. The isolate, which was …