The protonation state of soluble and membrane-associated macromolecules dictates their
charge, conformation, and functional activity. In addition, protons (H+ or their equivalents) …

Highlights•Transcytosis is actively suppressed in CNS endothelial cells for barrier
integrity.•Transcytosis is dynamically regulated during development and …

Calcium phosphates (CaPs) are the most widely used bone substitutes in bone tissue
engineering due to their compositional similarities to bone mineral and excellent …

To study charge-dependent interactions of nanoparticles (NPs) with biological media and
NP uptake by cells, colloidal gold nanoparticles were modified with amphiphilic polymers to …

A quantitative method is described for the measurement of intralysosomal pH in living cells.
Fluorescein isothiocyanate-labeled dextran (FD) is endocytized and accumulates in …

Mammalian cells take up extracellular material by a variety of different mechanisms that are
collectively termed endocytosis. Endocytic mechanisms serve many important cellular …

At pH 5.4 apotransferrin (iron-free transferrin) binds to cell-surface transferrin receptors to
the same extent and with the same affinity as does diferric transferrin at pH 7.0 …

Despite the critical need for iron in many cellular reactions, deletion of the transferrin
pathway does not block organogenesis, suggesting the presence of alternative methods to …

The study of endocytosis has traditionally focused on the contents of endocytic vacuoles, ie,
extracellular fluid, dissolved solutes, and macromolecules or particles which specifically or …

Tumor specific drug delivery has become increasingly interesting in cancer therapy, as the
use of chemotherapeutics is often limited due to severe side effects. Conventional drug …