Silicon has generally not been considered essential for plant growth, although it is well
recognized that many plants, particularly Poaceae, have substantial plant tissue …

This article reviews the development of immobilized-metal affinity chromatography (IMAC)
and describes its most important applications. We provide an overview on the use of IMAC in …

This protocol describes the use of TurboID and split-TurboID in proximity labeling
applications for map** protein–protein interactions and subcellular proteomes in live …

Gel-based proteomics is one of the most versatile methods for fractionating protein
complexes. Among these methods, two dimensional-polyacrylamide gel electrophoresis (2 …

One of the most important early developments in the field of proteomics was the advent of
automated data acquisition routines that allowed high‐throughput unattended data …

Proteins are the key players in many cellular processes. Their composition, trafficking, and
interactions underlie the dynamic processes of life. Furthermore, diseases are frequently …

Biological membranes form an essential barrier between living cells and their external
environments, as well as serve to compartmentalize intracellular organelles within …

Proteomic analyses of different subcellular compartments, so-called organellar proteomics,
facilitate the understanding of cellular functions on a molecular level. In this work, various …

Cell wall proteins are essential constituents of plant cell walls; they are involved in
modifications of cell wall components, wall structure, signaling and interactions with plasma …

Dysregulated protein synthesis is a core pathogenic mechanism in Fragile X Syndrome (FX).
The mGluR Theory of FX predicts that pathological synaptic changes arise from the …