This paper reviews the rapid advances that have been made in one form of optical biological
imaging in the last decade, namely that of light sheet microscopy. Although the concept was …

Light-sheet fluorescence microscopy (LSFM) methods collectively represent the major
breakthrough in developmental bio-imaging of living multicellular organisms. They are …

Light-sheet imaging of cleared and expanded samples creates terabyte-sized datasets that
consist of many unaligned three-dimensional image tiles, which must be reconstructed …

The Drosophila genome contains> 13000 protein-coding genes, the majority of which
remain poorly investigated. Important reasons include the lack of antibodies or reporter …

Rationale: microRNAs (miRNAs) modulate gene expression by repressing translation of
targeted genes. Previous work has established a role for miRNAs in regulating vascular …

During development, coordinated cell behaviors orchestrate tissue and organ
morphogenesis. Detailed descriptions of cell lineages and behaviors provide a powerful …

During gastrulation, physical forces reshape the simple embryonic tissue to form the
complex body plans of multicellular organisms. These forces often cause large-scale …

The development of fluorescent labels and powerful imaging technologies in the last two
decades has revolutionized the field of fluorescence microscopy, which is now widely used …

Many animal embryos pull and close an epithelial sheet around the ellipsoidal egg surface
during a gastrulation process known as epiboly. The ovoidal geometry dictates that the …

In higher plants, germline differentiation occurs during a relatively short period within
develo** flowers. Understanding of the mechanisms that govern germline differentiation …