[HTML][HTML] Clarifying tissue clearing
Biological specimens are intrinsically three dimensional; however, because of the obscuring
effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to …
effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to …
Cosolvent effects on protein stability
Proteins are marginally stable, and the folding/unfolding equilibrium of proteins in aqueous
solution can easily be altered by the addition of small organic molecules known as …
solution can easily be altered by the addition of small organic molecules known as …
Supramolecular thermo‐contracting adhesive hydrogel with self‐removability simultaneously enhancing noninvasive wound closure and MRSA‐infected wound …
R Yu, M Li, Z Li, G Pan, Y Liang… - Advanced Healthcare …, 2022 - Wiley Online Library
Conventional wound closure and dressing are two crucial, time‐consuming but isolated
principles in wound care. Even though tissue adhesive opens a new era for wound closure …
principles in wound care. Even though tissue adhesive opens a new era for wound closure …
Mechanisms of protein stabilization and prevention of protein aggregation by glycerol
V Vagenende, MGS Yap, BL Trout - Biochemistry, 2009 - ACS Publications
The stability of proteins in aqueous solution is routinely enhanced by cosolvents such as
glycerol. Glycerol is known to shift the native protein ensemble to more compact states …
glycerol. Glycerol is known to shift the native protein ensemble to more compact states …
Protein–excipient interactions: Mechanisms and biophysical characterization applied to protein formulation development
TJ Kamerzell, R Esfandiary, SB Joshi… - Advanced drug delivery …, 2011 - Elsevier
The purpose of this review is to demonstrate the critical importance of understanding protein–
excipient interactions as a key step in the rational design of formulations to stabilize and …
excipient interactions as a key step in the rational design of formulations to stabilize and …
Chemistry of Hofmeister anions and osmolytes
The study of the interactions of salts and osmolytes with macromolecules in aqueous
solution originated with experiments concerning protein precipitation more than 100 years …
solution originated with experiments concerning protein precipitation more than 100 years …
Chemical principles in tissue clearing and staining protocols for whole-body cell profiling
K Tainaka, A Kuno, SI Kubota… - Annual review of cell …, 2016 - annualreviews.org
Mammalian bodies have more than a billion cells per cubic centimeter, which makes whole-
body cell (WBC) profiling of an organism one of the ultimate challenges in biology and …
body cell (WBC) profiling of an organism one of the ultimate challenges in biology and …
Physical and chemical mechanisms of tissue optical clearing
T Yu, J Zhu, D Li, D Zhu - Iscience, 2021 - cell.com
Advanced optical methods combined with various probes pave the way toward molecular
imaging within living cells. However, major challenges are associated with the need to …
imaging within living cells. However, major challenges are associated with the need to …
Water structure and chaotropicity: their uses, abuses and biological implications
P Ball, JE Hallsworth - Physical Chemistry Chemical Physics, 2015 - pubs.rsc.org
The concept of “water structure” has been invoked to explain all manner of aqueous
phenomena. Here we look at the origins of this tendency to understand solute hydration in …
phenomena. Here we look at the origins of this tendency to understand solute hydration in …
Urea, but not guanidinium, destabilizes proteins by forming hydrogen bonds to the peptide group
WK Lim, J Rösgen… - Proceedings of the …, 2009 - National Acad Sciences
The mechanism by which urea and guanidinium destabilize protein structure is
controversial. We tested the possibility that these denaturants form hydrogen bonds with …
controversial. We tested the possibility that these denaturants form hydrogen bonds with …