Biomolecular condensate formation has been implicated in a host of biological processes
and has found relevance in biology and disease. Understanding the physical principles and …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

Phase transitions are important to understand cell dynamics, and the maturation of liquid
droplets is relevant to neurodegenerative disorders. We combined NMR and Raman …

Liquid–liquid phase separation of flexible biomolecules has been identified as a ubiquitous
phenomenon underlying the formation of membraneless organelles that harbor a multitude …

Many biomolecular condensates are enriched in and depend on RNAs and RNA binding
proteins (RBPs). So far, only a few studies have addressed the characterization of the …

Distance distribution information obtained by pulsed dipolar EPR spectroscopy provides an
important contribution to many studies in structural biology. Increasingly, such information is …

Biomolecular condensates formed via phase separation of proteins and nucleic acids are
thought to be associated with a wide range of cellular functions and dysfunctions. We dissect …

Phase separation is a ubiquitous and emerging mechanism underlying intracellular
organization. Yet how distinct molecular compositions in phase-separated condensates are …

The electron–electron double resonance (DEER) method, which provides distance
distributions between two spin labels, attached site specifically to biomolecules (proteins …

Many membraneless organelles are liquid-like domains that form inside the active,
viscoelastic environment of living cells through phase separation. To investigate the …