Methods based on single-molecule localization and photophysics have brought nanoscale
imaging with visible light into reach. This has enabled single-particle tracking applications …

Advances in optical manipulation and observation of neural activity have set the stage for
widespread implementation of closed-loop and activity-guided optical control of neural …

Resolution in optical nanoscopy (or super-resolution microscopy) depends on the
localization uncertainty and density of single fluorescent labels and on the sample's spatial …

Abstract Extending three-dimensional (3D) single-molecule localization microscopy away
from the coverslip and into thicker specimens will greatly broaden its biological utility …

Super-resolution localization microscopy methods provide powerful new capabilities for
probing biology at the nanometer scale via fluorescence. These methods rely on two key …

Optical nanoscopy techniques using localization based image reconstruction, also termed
super-resolution microscopy (SRM), have become a standard tool to bypass the diffraction …

The localization precision is a crucial and important parameter for single-molecule
localization microscopy (SMLM) and directly influences the achievable spatial resolution. It …

Validating and testing a fluorescence microscope or a microscopy method requires defined
samples that can be used as standards. DNA origami is a new tool that provides a …

The recent 2014 Nobel Prize in chemistry honored an era of discoveries and technical
advancements in the field of super‐resolution microscopy. However, the applications of …

Super-resolution fluorescence microscopy allows the investigation of cellular structures at
nanoscale resolution using light. Current developments in super-resolution microscopy have …