Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification

MT Parker, K Knop, AV Sherwood, NJ Schurch… - Elife, 2020 - elifesciences.org
Understanding genome organization and gene regulation requires insight into RNA
transcription, processing and modification. We adapted nanopore direct RNA sequencing to …

In situ organic biosignature detection techniques for space applications

V Abrahamsson, I Kanik - Frontiers in Astronomy and Space Sciences, 2022 - frontiersin.org
The search for life in Solar System bodies such as Mars and Ocean Worlds (eg, Europa and
Enceladus) is an ongoing and high-priority endeavor in space science, even∼ five decades …

Relative performance of MinION (Oxford Nanopore Technologies) versus Sequel (Pacific Biosciences) third-generation sequencing instruments in identification of …

K Loit, K Adamson, M Bahram, R Puusepp… - Applied and …, 2019 - Am Soc Microbiol
Culture-based molecular identification methods have revolutionized detection of pathogens,
yet these methods are slow and may yield inconclusive results from environmental …

Subsurface science and search for life in ocean worlds

JD Lawrence, AD Mullen, FE Bryson… - The Planetary …, 2023 - iopscience.iop.org
Several worlds in our solar system are thought to hold oceans of liquid water beneath their
frozen surfaces. These subsurface ice and ocean environments are promising targets in the …

Enceladus as a potential oasis for life: Science goals and investigations for future explorations

G Choblet, G Tobie, A Buch, O Čadek, LM Barge… - Experimental …, 2022 - Springer
Enceladus is the first planetary object for which direct sampling of a subsurface water
reservoir, likely habitable, has been performed. Over a decade of flybys and seven …

The limits, capabilities, and potential for life detection with MinION sequencing in a Paleochannel Mars analog

C Maggiori, J Stromberg, Y Blanco, J Goordial… - Astrobiology, 2020 - liebertpub.com
No instrument capable of direct life detection has been included on a mission payload to
Mars since NASA's Viking missions in the 1970s. This prevents us from discovering whether …

Nucleic Acid Extraction and Sequencing from Low-Biomass Synthetic Mars Analog Soils for In Situ Life Detection

A Mojarro, J Hachey, R Bailey, M Brown, R Doebler… - Astrobiology, 2019 - liebertpub.com
Recent studies regarding the origins of life and Mars-Earth meteorite transfer simulations
suggest that biological informational polymers, such as nucleic acids (DNA and RNA), have …

Field-based detection of bacteria using nanopore sequencing: Method evaluation for biothreat detection in complex samples

AD Tyler, J McAllister, H Stapleton, P Gauci… - Plos one, 2023 - journals.plos.org
From pathogen detection to genome or plasmid closure, the utility of the Oxford Nanopore
Technologies (ONT) MinION for microbiological analysis has been well documented. The …

Applying Novel Techniques from Physical and Biological Sciences to Life Detection

TZ Jia, C Giri, A Aldaba, I Bahcivan… - Space: Science & …, 2023 - spj.science.org
One of the primary goals of the space exploration community is to unambiguously detect
past or present life outside of Earth. As such, a number of so-called life detection …

Nucleic acid sequencing under Mars-like conditions

CE Carr, K Saboda, A Mojarro… - 2019 IEEE …, 2019 - ieeexplore.ieee.org
All known life uses informational polymers based on nucleic acids. Future missions to Mars
and Ocean Worlds such as Enceladus and/or Europa may target these or related polymers …