Deuterium‐and tritium‐labelled compounds: applications in the life sciences

J Atzrodt, V Derdau, WJ Kerr… - Angewandte chemie …, 2018 - Wiley Online Library
Hydrogen isotopes are unique tools for identifying and understanding biological and
chemical processes. Hydrogen isotope labelling allows for the traceless and direct …

Metabolomics for plant stress response

V Shulaev, D Cortes, G Miller, R Mittler - Physiologia plantarum, 2008 - Wiley Online Library
Stress in plants could be defined as any change in growth condition (s) that disrupts
metabolic homeostasis and requires an adjustment of metabolic pathways in a process that …

Advances in structure elucidation of small molecules using mass spectrometry

T Kind, O Fiehn - Bioanalytical reviews, 2010 - Springer
The structural elucidation of small molecules using mass spectrometry plays an important
role in modern life sciences and bioanalytical approaches. This review covers different soft …

Applications of NMR spectroscopy to systems biochemistry

TWM Fan, AN Lane - Progress in nuclear magnetic resonance spectroscopy, 2016 - Elsevier
The past decades of advancements in NMR have made it a very powerful tool for metabolic
research. Despite its limitations in sensitivity relative to mass spectrometric techniques, NMR …

Elemental formula annotation of polar and lipophilic metabolites using 13C, 15N and 34S isotope labelling, in combination with high‐resolution mass spectrometry

P Giavalisco, Y Li, A Matthes, A Eckhardt… - The Plant …, 2011 - Wiley Online Library
The unbiased and comprehensive analysis of metabolites in any organism presents a major
challenge if proper peak annotation and unambiguous assignment of the biological origin of …

Differential 12C-/13C-Isotope Dansylation Labeling and Fast Liquid Chromatography/Mass Spectrometry for Absolute and Relative Quantification of the Metabolome

K Guo, L Li - Analytical chemistry, 2009 - ACS Publications
We report a new quantitative metabolome profiling technique based on differential 12C-/13C-
isotope dansylation labeling of metabolites, fast liquid chromatography (LC) separation and …

Stable isotope-labeling studies in metabolomics: new insights into structure and dynamics of metabolic networks

A Chokkathukalam, DH Kim, MP Barrett, R Breitling… - Bioanalysis, 2014 - Taylor & Francis
The rapid emergence of metabolomics has enabled system-wide measurements of
metabolites in various organisms. However, advances in the mechanistic understanding of …

Avoiding misannotation of in-source fragmentation products as cellular metabolites in liquid chromatography–mass spectrometry-based metabolomics

YF Xu, W Lu, JD Rabinowitz - Analytical chemistry, 2015 - ACS Publications
Liquid chromatography–mass spectrometry (LC-MS) technology allows for rapid quantitation
of cellular metabolites, with metabolites identified by mass spectrometry and …

Metabolomic technologies and their application to the study of plants and plant–host interactions

JW Allwood, DI Ellis, R Goodacre - Physiologia plantarum, 2008 - Wiley Online Library
Metabolomics is perhaps the ultimate level of post‐genomic analysis as it can reveal
changes in metabolite fluxes that are controlled by only minor changes within gene …

Ultra performance liquid chromatography and high resolution mass spectrometry for the analysis of plant lipids

J Hummel, S Segu, Y Li, S Irgang, J Jueppner… - Frontiers in plant …, 2011 - frontiersin.org
Holistic analysis of lipids is becoming increasingly popular in the life sciences. Recently,
several interesting, mass spectrometry-based studies have been conducted, especially in …