CRISPR/Cas9 technology has revolutionized biology. This prokaryotic defense system
against foreign DNA has been repurposed for genome editing in a broad range of cell …

Few genetic tools were available to work with Trypanosoma cruzi until the recent
introduction of the CRISPR/Cas9 technique for gene knockout, gene knock-in, gene …

Trypanosoma cruzi is a protist parasite and the causative agent of American
trypanosomiasis or Chagas disease. The parasite life cycle in its mammalian host includes …

Leishmania parasites include important pathogens and model organisms and are even
used for the production of recombinant proteins. However, functional genomics and the …

Nowadays, most reverse genetics approaches in Trypanosoma brucei, a protozoan parasite
of medical and veterinary importance, rely on pre-established cell lines. Consequently …

Membrane proteins in trypanosomatids are, in general, weakly expressed and confirmation
of their subcellular localization frequently requires their overexpression with epitope tags …

Trypanosoma cruzi is an obligate intracellular parasite that is the causative agent for
Chagas Disease, a chronic bloodborne infection endemic to the Americas. Inositol …

Compared to canonical model organisms, the genetic toolbox of Kinetoplastid parasites
have a considerable gap in the transgenic techniques available. The implementation of the …

Inducibilní expresní systémy jsou systémy, které umožňují ‚zapínat'a ‚vypínat'expresi genu
zájmu, a jsou tedy užitečným nástrojem pro studium funkce genů. Existují desítky různých …