In the realm of biological research, the invention of super-resolution microscopy (SRM) has
enabled the visualization of ultrafine sub-cellular structures and their functions in live cells at …

Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and
cellular structures at the nanometer scale. Employing these techniques in living cells has …

Recently, click chemistry has provided important advances in biomedical research fields.
Particularly, copper-free click chemistry including strain-promoted azide–alkyne …

In this Primer, we focus on the most recent advancements in stimulated emission depletion
(STED) microscopy, encompassing optics, computational microscopy and probes design …

Advancement of bioorthogonal chemistry in molecular optical imaging lies in expanding the
repertoire of fluorophores that can undergo fluorescence signal changes upon …

Fluorescence microscopy has become a critical tool for researchers to understand biological
processes at the cellular level. Micrographs from fixed and live-cell imaging procedures …

The proof of concept for conditionally activatable photocages is demonstrated on a new
vinyltetrazine-derivatized coumarin. The tetrazine form is disabled in terms of light-induced …

Fluorescence imaging is an invaluable tool to study biological processes and further
progress depends on the development of advanced fluorogenic probes that reach …

Fluorogenic probes efficiently reduce non-specific background signals, which often results in
highly improved signal-to-noise ratios. Although this implies improved resolution …

Genetic code expansion (GCE) technology allows the specific incorporation of
functionalized noncanonical amino acids (ncAAs) into proteins. Here, we investigated the …