Second harmonic generation (SHG) microscopy is an important optical imaging technique in
a variety of applications. This article describes the history and physical principles of SHG …

The assembly of the mitotic spindle and the subsequent segregation of sister chromatids are
based on the self-organized action of microtubule filaments, motor proteins, and other …

STUDY QUESTION Is the combined use of fluorescence lifetime imaging microscopy (FLIM)-
based metabolic imaging and second harmonic generation (SHG) spindle imaging a …

Cytoskeletal networks are foundational examples of active matter and central to self-
organized structures in the cell. In vivo, these networks are active and densely crosslinked …

Fibrosis can affect any organ, resulting in the loss of tissue architecture and function with
often life-threatening consequences. Pathologically, fibrosis is characterised by the …

Microtubules are a vital component of the cell's cytoskeleton and their organization is crucial
for healthy cell functioning. The use of label-free SH imaging of microtubules remains …

In microtubule-based active nematics, motor-driven extensile motion of microtubule bundles
powers chaotic large-scale dynamics. We quantify the interfilament sliding motion both in …

STUDY QUESTION Can non-invasive metabolic imaging via fluorescence lifetime imaging
microscopy (FLIM) detect variations in metabolic profiles between discarded human …

Microtubules, maintaining a non-linear structure, are suitable for direct observation in living
mammalian by second-harmonic imaging microscopy (SHIM)(a new kind of confocal …

Polarization-resolved nonlinear optical microscopy can provide label-free images of
anisotropic biomaterials. Existing techniques are often limited by their complexity …