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[HTML][HTML] Therapeutic glycoprotein production in mammalian cells
Over the last years, the biopharmaceutical industry has significantly turned its biologics
production towards mammalian cell expression systems. The presence of glycosylation …
production towards mammalian cell expression systems. The presence of glycosylation …
[HTML][HTML] Perfusion mammalian cell culture for recombinant protein manufacturing–A critical review
The manufacturing of recombinant protein is traditionally divided in two main steps:
upstream (cell culture and synthesis of the target protein) and downstream (purification and …
upstream (cell culture and synthesis of the target protein) and downstream (purification and …
[HTML][HTML] Trends in upstream and downstream process development for antibody manufacturing
P Gronemeyer, R Ditz, J Strube - Bioengineering, 2014 - mdpi.com
A steady increase of product titers and the corresponding change in impurity composition
represent a challenge for development and optimization of antibody production processes …
represent a challenge for development and optimization of antibody production processes …
Gene expression in mammalian cells and its applications
KH Khan - Advanced pharmaceutical bulletin, 2013 - pmc.ncbi.nlm.nih.gov
The production of proteins in appropriate quantity and quality is an essential requirement of
the present time. There appears to be a progressive increase in the application of …
the present time. There appears to be a progressive increase in the application of …
Advances in the production and downstream processing of antibodies
Sales of monoclonal antibody (mAbs) therapies exceeded
40billionin2010andareexpectedtoreach 70 billion by 2015. The majority of the approved …
40billionin2010andareexpectedtoreach 70 billion by 2015. The majority of the approved …
Size matters for in vitro gene delivery: investigating the relationships among complexation protocol, transfection medium, size and sedimentation
Although branched and linear polyethylenimines (bPEIs and lPEIs) are gold standard
transfectants, a systematic analysis of the effects of the preparation protocol of polyplexes …
transfectants, a systematic analysis of the effects of the preparation protocol of polyplexes …
Improving the efficiency of CHO cell line generation using glutamine synthetase gene knockout cells
L Fan, I Kadura, LE Krebs, CC Hatfield… - Biotechnology and …, 2012 - Wiley Online Library
Although Chinese hamster ovary (CHO) cells, with their unique characteristics, have
become a major workhorse for the manufacture of therapeutic recombinant proteins, one of …
become a major workhorse for the manufacture of therapeutic recombinant proteins, one of …
IRES-mediated Tricistronic vectors for enhancing generation of high monoclonal antibody expressing CHO cell lines
A Tricistronic vector utilizing internal ribosome entry site (IRES) elements to express the light
chain (LC), heavy chain (HC), and a neomycin phosphotransferase (NPT) selection marker …
chain (LC), heavy chain (HC), and a neomycin phosphotransferase (NPT) selection marker …
Comprehensive characterization of glutamine synthetase-mediated selection for the establishment of recombinant CHO cells producing monoclonal antibodies
To characterize a glutamine synthetase (GS)-based selection system, monoclonal antibody
(mAb) producing recombinant CHO cell clones were generated by a single round of …
(mAb) producing recombinant CHO cell clones were generated by a single round of …
The emerging role of systems biology for engineering protein production in CHO cells
Highlights•3 waves of innovation have enhanced protein production in CHO cells.•Systems-
level methods are now unravelling drivers of protein production.•Predictive models will …
level methods are now unravelling drivers of protein production.•Predictive models will …