Automated chemical oligosaccharide synthesis: novel approach to traditional challenges

M Panza, SG Pistorio, KJ Stine… - Chemical reviews, 2018 - ACS Publications
Advances in carbohydrate chemistry have certainly made common oligosaccharides much
more accessible. However, many current methods still rely heavily upon specialized …

Recent advances in the analysis of complex glycoproteins

S Gaunitz, G Nagy, NLB Pohl, MV Novotny - Analytical chemistry, 2017 - ACS Publications
Notes 408 Biographies 408 Acknowledgments 409 References 409 The enormous
structural diversity of glycoconjugates mirrors their myriad biological functions in prokaryotic …

Recent liquid chromatographic approaches and developments for the separation and purification of carbohydrates

G Nagy, T Peng, NLB Pohl - Analytical Methods, 2017 - pubs.rsc.org
Carbohydrate purification remains a bottleneck in securing analytical standards from natural
sources or by chemical or enzymatic synthesis. This review highlights the scope and …

Purification and concentration of minor impurity in the bulk drug by step-gradient twin-column recycling chromatography

F Wei, Y Hu, Y Zhao, Y Wu, G ** - Industrial & Engineering …, 2023 - ACS Publications
A weaker solvent was constantly added at the middle of the two columns to set up a two-step
solvent gradient in twin-column recycling chromatography so that the eluotropic strength of …

Structural elucidation approaches in carbohydrates: A comprehensive review on techniques and future trends

Y Liu, Y Huang, R Zhu, MA Farag, E Capanoglu… - Food Chemistry, 2023 - Elsevier
Carbohydrates and their implications for human health have been the subject to a rapidly
growing interest. Substantial advances in analytical methods have enabled a more effective …

HPLC‐Based Automated Synthesis of Glycans in Solution

S Escopy, Y Singh, KJ Stine… - Chemistry–A European …, 2022 - Wiley Online Library
As the 21st century unfolds with rapid changes, new challenges in research and
development emerge. These new challenges prompted us to repurpose our HPLC− A …

Recycling Preparative Liquid Chromatography, the Overlooked Methodology for the Purification of Natural Products

R Pereda-Miranda, JF Castañeda-Gómez… - Revista Brasileira de …, 2024 - Springer
Frequently, preparative high-performance liquid chromatography separations of complex
natural product mixtures by adsorption chromatography are erratic to achieve full baseline …

Rebirth of recycling liquid chromatography with modern chromatographic columns: Extension to gradient elution

F Gritti - Journal of Chromatography A, 2021 - Elsevier
Twin column recycling semi-preparative liquid chromatography (TCRLC) is revived to
prepare small amount (∼ 1 mg) of a pure targeted compound, which cannot be isolated by …

Power law approach as a convenient protocol for improving peak shapes and recovering areas from partially resolved peaks

MF Wahab, F Gritti, TC O'Haver, G Hellinghausen… - Chromatographia, 2019 - Springer
Separation techniques have developed rapidly where sub-second chromatography,
ultrahigh resolution recycling chromatography, and two-dimensional liquid chromatography …

Resolution limits of size exclusion chromatography columns identified from flow reversal and overcome by recycling liquid chromatography to improve the …

F Gritti - Journal of Chromatography A, 2023 - Elsevier
The flow reversal (FR) technique consists of reversing the flow direction along a
chromatographic column. It is used to reveal the origin (such as poor column packing, active …