CRISPR/Cas-based nucleic acid detection strategies: Trends and challenges

J Zhou, Z Li, JS Olajide, G Wang - Heliyon, 2024 - cell.com
CRISPR/Cas systems have become integral parts of nucleic acid detection apparatus and
biosensors. Various CRISPR/Cas systems such as CRISPR/Cas9, CRISPR/Cas12 …

Recent progress in nucleic acid detection with CRISPR

FX Liu, JQ Cui, Z Wu, S Yao - Lab on a Chip, 2023 - pubs.rsc.org
Recent advances in CRISPR-based biotechnologies have greatly expanded our capabilities
to repurpose CRISPR for the development of molecular diagnostic systems. The key …

Pneumatic nano-sieve for CRISPR-based detection of drug-resistant bacteria

R Peng, X Chen, F Xu, R Hailstone, Y Men, K Du - Nanoscale horizons, 2023 - pubs.rsc.org
The increasing prevalence of antibiotic-resistant bacterial infections, particularly methicillin-
resistant Staphylococcus aureus (MRSA), presents a significant public health concern …

[HTML][HTML] Current and perspective sensing methods for monkeypox virus

I Gul, C Liu, X Yuan, Z Du, S Zhai, Z Lei, Q Chen… - Bioengineering, 2022 - mdpi.com
The outbreak of the monkeypox virus (MPXV) in non-endemic countries is an emerging
global health threat and may have an economic impact if proactive actions are not taken. As …

A perspective on computer vision in biosensing

L Liu, K Du - Biomicrofluidics, 2024 - pubs.aip.org
Computer vision has become a powerful tool in the field of biosensing, aiding in the
development of innovative and precise systems for the analysis and interpretation of …

Micro-and nanosystems for the detection of hemorrhagic fever viruses

M Bao, J Waitkus, L Liu, Y Chang, Z Xu, P Qin, J Chen… - Lab on a Chip, 2023 - pubs.rsc.org
Hemorrhagic fever viruses (HFVs) are virulent pathogens that can cause severe and often
fatal illnesses in humans. Timely and accurate detection of HFVs is critical for effective …

CRISPR/Cas12a triggered SERS and naked eye dual-mode biosensor for ultrasensitive and on-site detection of nucleic acid via cascade signal amplification

J Liu, J Chen, S Jia, Y Wang, D Wu, Y Wu… - Sensors and Actuators B …, 2024 - Elsevier
Highly sensitive and on-site detection of nucleic acids has always been a critical issue in the
field of analytical chemistry. Surface-enhanced Raman scattering (SERS)-based biosensing …

Cleavable energy transfer labeled oligonucleotide probe for enhanced isothermal amplification detection and nano digital chip-based readout

L Liu, SJ Dollery, GJ Tobin, G Lu, K Du - Nanoscale, 2025 - pubs.rsc.org
Quantitative analysis of human papillomavirus (HPV)-infected cervical cancer is essential for
early diagnosis and timely treatment of cervical cancer. Here, we introduce a novel energy …

Emerging microfluidic technologies for CRISPR-based diagnostics: an overview

F Nafian, KS Esfahani, MH Aghmiuni, S Khoushab… - Analytical …, 2025 - pubs.rsc.org
In recent years, CRISPR (clustered regularly interspaced short palindromic repeats) has
emerged as a detection technique with high specificity and sensitivity. However, it still needs …

[HTML][HTML] Instrument-Free Point-of-Care Diagnostic for Leishmania Parasites

TJ Wiggins, R Peng, RV Bushnell, JK Tobin… - Diagnostics, 2024 - mdpi.com
Background/Objective: Leishmaniasis is the second deadliest parasitic disease in the world,
after malaria, with an estimated 1.6 million new cases each year. While cutaneous …