Formative pluripotency: the executive phase in a developmental continuum
A Smith - Development, 2017 - journals.biologists.com
The regulative capability of single cells to give rise to all primary embryonic lineages is
termed pluripotency. Observations of fluctuating gene expression and phenotypic …
termed pluripotency. Observations of fluctuating gene expression and phenotypic …
A double take on bivalent promoters
Histone modifications and chromatin-associated protein complexes are crucially involved in
the control of gene expression, supervising cell fate decisions and differentiation. Many …
the control of gene expression, supervising cell fate decisions and differentiation. Many …
Rolling back human pluripotent stem cells to an eight-cell embryo-like stage
After fertilization, the quiescent zygote experiences a burst of genome activation that initiates
a short-lived totipotent state. Understanding the process of totipotency in human cells would …
a short-lived totipotent state. Understanding the process of totipotency in human cells would …
Capture of mouse and human stem cells with features of formative pluripotency
Pluripotent cells emerge as a naive founder population in the blastocyst, acquire capacity for
germline and soma formation, and then undergo lineage priming. Mouse embryonic stem …
germline and soma formation, and then undergo lineage priming. Mouse embryonic stem …
[HTML][HTML] Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells
It has long been the dream of biologists to map gene expression at the single-cell level. With
such data one might track heterogeneous cell sub-populations, and infer regulatory …
such data one might track heterogeneous cell sub-populations, and infer regulatory …
Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity
We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to
accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells …
accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells …
Single cell RNA-sequencing of pluripotent states unlocks modular transcriptional variation
Embryonic stem cell (ESC) culture conditions are important for maintaining long-term self-
renewal, and they influence cellular pluripotency state. Here, we report single cell RNA …
renewal, and they influence cellular pluripotency state. Here, we report single cell RNA …
Chromatin modifiers and remodellers: regulators of cellular differentiation
T Chen, SYR Dent - Nature Reviews Genetics, 2014 - nature.com
Cellular differentiation is, by definition, epigenetic. Genome-wide profiling of pluripotent cells
and differentiated cells suggests global chromatin remodelling during differentiation, which …
and differentiated cells suggests global chromatin remodelling during differentiation, which …
Embryonic stem cell potency fluctuates with endogenous retrovirus activity
TS Macfarlan, WD Gifford, S Driscoll, K Lettieri… - Nature, 2012 - nature.com
Embryonic stem (ES) cells are derived from blastocyst-stage embryos and are thought to be
functionally equivalent to the inner cell mass, which lacks the ability to produce all …
functionally equivalent to the inner cell mass, which lacks the ability to produce all …
[HTML][HTML] Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells
The generation of properly functioning gametes in vitro requires reconstitution of the
multistepped pathway of germ cell development. We demonstrate here the generation of …
multistepped pathway of germ cell development. We demonstrate here the generation of …