Engineering of thermostable enzymes for industrial applications
The catalytic properties of some selected enzymes have long been exploited to carry out
efficient and cost-effective bioconversions in a multitude of research and industrial sectors …
efficient and cost-effective bioconversions in a multitude of research and industrial sectors …
Insights from molecular dynamics simulations for computational protein design
A grand challenge in the field of structural biology is to design and engineer proteins that
exhibit targeted functions. Although much success on this front has been achieved, design …
exhibit targeted functions. Although much success on this front has been achieved, design …
Modular binder technology by NGS-aided, high-resolution selection in yeast of designed armadillo modules
Establishing modular binders as diagnostic detection agents represents a cost-and time-
efficient alternative to the commonly used binders that are generated one molecule at a time …
efficient alternative to the commonly used binders that are generated one molecule at a time …
Unexpected dynamics in femtomolar complexes of binding proteins with peptides
Ultra-tight binding is usually observed for proteins associating with rigidified molecules.
Previously, we demonstrated that femtomolar binders derived from the Armadillo repeat …
Previously, we demonstrated that femtomolar binders derived from the Armadillo repeat …
Modern computational methods for rational enzyme engineering
Biocatalysis is a thriving field of research because of the vast, environmentally friendly
impact that enzymes have on industry and biotechnology. However, in most production …
impact that enzymes have on industry and biotechnology. However, in most production …
Toxin neutralization using alternative binding proteins
Animal toxins present a major threat to human health worldwide, predominantly through
snakebite envenomings, which are responsible for over 100,000 deaths each year. To date …
snakebite envenomings, which are responsible for over 100,000 deaths each year. To date …
The emerging role of new protein scaffold-based agents for treatment of cancer
UH Weidle, J Auer, U Brinkmann… - Cancer genomics & …, 2013 - cgp.iiarjournals.org
In order to overcome limitations of monoclonal antibodies, new protein-based scaffolds have
been designed and evaluated pre-clinically, and some of them are in clinical studies for the …
been designed and evaluated pre-clinically, and some of them are in clinical studies for the …
Designing repeat proteins: a modular approach to protein design
Highlights•Modular architecture of repeat proteins reduces design complexity.•Idealized self-
compatible units of native families are designed using sequence and structural information …
compatible units of native families are designed using sequence and structural information …
Designed Armadillo repeat proteins: library generation, characterization and selection of peptide binders with high specificity
G Varadamsetty, D Tremmel, S Hansen… - Journal of molecular …, 2012 - Elsevier
Designed Armadillo repeat proteins (ArmRPs) are a novel class of binding proteins intended
for general modular peptide binding and have very favorable expression and stability …
for general modular peptide binding and have very favorable expression and stability …
Modular peptide binding: from a comparison of natural binders to designed armadillo repeat proteins
C Reichen, S Hansen, A Plückthun - Journal of structural biology, 2014 - Elsevier
Several binding scaffolds that are not based on immunoglobulins have been designed as
alternatives to traditional monoclonal antibodies. Many of them have been developed to …
alternatives to traditional monoclonal antibodies. Many of them have been developed to …