Super-resolution microscopy provides direct insight into fundamental biological processes
occurring at length scales smaller than light's diffraction limit. The analysis of data at such …

Coding for proteins has been considered the main function of RNA since the “central
dogma” of biology was proposed. The discovery of noncoding transcripts shed light on …

RNA molecules play vital roles in many cellular processes. Visualising their dynamics in live
cells at single-molecule resolution is essential to elucidate their role in RNA metabolism …

Nanoparticles are a promising solution for delivery of a wide range of medicines and
vaccines. Optimizing their design depends on being able to resolve, understand, and predict …

The stoichiometry of protein complexes is precisely regulated in cells and is fundamental to
protein function. Singe-molecule fluorescence imaging based photobleaching event …

Single-molecule techniques allow the visualization of the molecular dynamics of nucleic
acids and proteins with high spatiotemporal resolution. Valuable kinetic information of …

Fluorescence correlation spectroscopy (FCS) is a flexible and widely used tool routinely
exploited in vivo. While FCS provides estimates of dynamical quantities, such as diffusion …

The development of effective methods for the detection of protein misfolding is highly
beneficial for early stage medical diagnosis and the prevention of many neurodegenerative …

Fundamental properties of light unavoidably impose features on images collected using
fluorescence microscopes. Accounting for these features is often critical in quantitatively …

The counting of discrete photobleaching steps in fluorescence microscopy is ideally suited
to study protein complex stoichiometry in situ. The counting range of photobleaching step …