The blood–brain barrier (BBB) greatly restricts the entry of biological and engineered
therapeutic molecules into the brain. Due to challenges in translating results from animal …

Tissue clearing of gross anatomical samples was first described more than a century ago
and has only recently found widespread use in the field of microscopy. This renaissance has …

Aging results in gray and white matter degeneration, but the specific microglial responses
are unknown. Using single-cell RNA sequencing from white and gray matter separately, we …

The targeted degradation of membrane proteins would afford an attractive and general
strategy for treating various diseases that remain difficult with the current proteolysis …

Nanobodies are the recombinant variable domains of heavy-chain-only antibodies, with
many unique properties such as small size, excellent solubility, superior stability, quick …

Abstract Three-dimensional (3D) reconstruction of living brain tissue down to an individual
synapse level would create opportunities for decoding the dynamics and structure–function …

Purpose The aim of the present paper is to review the role of HER2 antibodies, affibodies
and nanobodies as vehicles for imaging and therapy approaches in breast cancer, including …

Resolving the distribution of specific proteins at the nanoscale in the ultrastructural context of
the cell is a major challenge in fluorescence microscopy. We report the discovery of a new …

Advances in light-sheet and confocal microscopy now allow imaging of cleared large
biological tissue samples and enable the 3D appreciation of cell and protein localization in …

For treatment and diagnosis of cancer, antibodies have proven their value and now serve as
a first line of therapy for certain cancers. A unique class of antibody fragments called …