UniProt is an important collection of protein sequences and their annotations, which has
doubled in size to 80 million sequences during the past year. This growth in sequences has …

Our understanding of metabolism is limited by a lack of knowledge about the functions of
many enzymes. Here, we develop a high-throughput mass spectrometry approach to …

Plant natural products (PNPs) and their derivatives are important but underexplored sources
of pharmaceutical molecules. To access this untapped potential, the reconstitution of …

Abstract The emergence of Next Generation Sequencing generates an incredible amount of
sequence and great potential for new enzyme discovery. Despite this huge amount of data …

Thousands of biochemical reactions with characterized activities are “orphan,” meaning they
cannot be assigned to a specific enzyme, leaving gaps in metabolic pathways. Novel …

Metabolic engineering uses microorganisms to synthesize chemicals from renewable
resources. Given the thousands of known metabolites, it is unclear what valuable chemicals …

Abstract Rhea (http://www. ebi. ac. uk/rhea) is a comprehensive and non-redundant
resource of expert-curated biochemical reactions described using species from the ChEBI …

Mono-ethylene glycol (MEG) is an important petrochemical with widespread use in
numerous consumer products. The current industrial MEG-production process relies on non …

Pseudomonas aeruginosa, Yersinia pestis, and many other bacteria are able to utilize the
C5-dicarboxylic acid itaconate (methylenesuccinate). Itaconate degradation starts with its …

The C. elegans gene swip-10 encodes an orphan metallo β-lactamase that genetic studies
indicate is vital for limiting neuronal excitability and viability. Sequence analysis indicates …