Rapid and accurate molecular diagnosis is a prerequisite for precision medicine, food
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …

Nucleic acid cleavage processes contribute to target and signal amplification, which is
critical for the development of highly sensitive assays. 1, 2 Exonucleases or endonucleases …

The pandemic of COVID-19 creates an imperative need for sensitive and portable detection
of SARS-CoV-2. We devised a SERS-read, CRISPR/Cas-powered nanobioassay, termed as …

The CRISPR-Cas12a system has revolutionized nucleic acid testing (NAT) with its rapid and
precise capabilities, yet it traditionally required RNA pre-amplification. Here we develop …

Bacterial abortive-infection systems limit the spread of foreign invaders by shutting down or
killing infected cells before the invaders can replicate 1, 2. Several RNA-targeting CRISPR …

Cas12a, a CRISPR-associated protein complex, has an inherent ability to cleave DNA
substrates and is utilized in diagnostic tools to identify DNA molecules. We demonstrate that …

Nucleic acid detection powered by CRISPR technology provides a rapid, sensitive, and
deployable approach to molecular diagnostics. While exciting, there remain challenges …

Strategies utilizing the CRISPR/Cas nucleases Cas13 and Cas12 have shown great
promise in the development of highly sensitive and rapid diagnostic assays for the detection …

Nucleic acid detection methods based on CRISPR and isothermal amplification techniques
show great potential for point-of-care diagnostic applications. However, most current …

Sensitive and specific assay of microRNAs (miRNAs) is beneficial to early disease
screening. Herein, we for the first time proposed clustered regularly interspaced short …