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CRISPR/Cas-powered amplification-free detection of nucleic acids: current state of the art, challenges, and futuristic perspectives
Y Li, Y Liu, X Tang, J Qiao, J Kou, S Man, L Zhu… - ACS …, 2023 - ACS Publications
CRISPR/Cas system is becoming an increasingly influential technology that has been
repositioned in nucleic acid detection. A preamplification step is usually required to improve …
repositioned in nucleic acid detection. A preamplification step is usually required to improve …
CRISPR/Cas and argonaute-based biosensors for pathogen detection
Z Yang, S Mao, L Wang, S Fu, Y Dong… - ACS …, 2023 - ACS Publications
Over the past few decades, pathogens have posed a threat to human security, and rapid
identification of pathogens should be one of the ideal methods to prevent major public …
identification of pathogens should be one of the ideal methods to prevent major public …
Argonaute-DNAzyme tandem biosensing for highly sensitive and simultaneous dual-gene detection of methicillin-resistant Staphylococcus aureus
Y Li, X Tang, N Wang, Z Zhao, S Man, L Zhu… - Biosensors and …, 2024 - Elsevier
Methicillin-resistant Staphylococcus aureus (MRSA), a common zoonotic multidrug-resistant
bacterium, puts a great threat to public health and food safety. Rapid and reliable detection …
bacterium, puts a great threat to public health and food safety. Rapid and reliable detection …
CRISPR-powered strategies for amplification-free diagnostics of infectious diseases
Y **a, R Rao, M **ong, B He, B Zheng, Y Jia… - Analytical …, 2024 - ACS Publications
Infectious diseases continue to present significant challenges globally, with high mortality
rates and escalating healthcare costs. The Clustered Regularly Interspaced Short …
rates and escalating healthcare costs. The Clustered Regularly Interspaced Short …
Next-generation pathogen detection: Exploring the power of nucleic acid amplification-free biosensors
Y Wang, Z Wang, Y Shang, J Wang, Z Zhu, L **… - Coordination Chemistry …, 2024 - Elsevier
Abstract Accurate, rapid, cost-effective, and point-of-care pathogen identification is crucial for
public health safety and encompasses disease prevention, food safety, and environmental …
public health safety and encompasses disease prevention, food safety, and environmental …
A portable deep-learning-assisted digital single-particle counting biosensing platform for amplification-free nucleic acid detection using a lens-free holography …
Y Zhou, J Zhao, R Chen, P Lu, W Zhao, R Ma, T **ao… - Nano Today, 2024 - Elsevier
The digital single-particle assay has emerged as a highly promising technology in various
detection applications, such as food safety inspection, environmental monitoring, and in vitro …
detection applications, such as food safety inspection, environmental monitoring, and in vitro …
One-pot isothermal amplification permits recycled activation of CRISPR/Cas12a for sensing terminal deoxynucleotidyl transferase activity
This study introduces a one-pot isothermal amplification assay for ultrasensitive analysis of
terminal deoxynucleotidyl transferase (TdT) activity. The system realizes recycled activation …
terminal deoxynucleotidyl transferase (TdT) activity. The system realizes recycled activation …
Ultrasensitive ImmunoMag-CRISPR lateral flow assay for point-of-care testing of urinary biomarkers
Rapid, accurate, and noninvasive detection of biomarkers in saliva, urine, or nasal fluid is
essential for the identification, early diagnosis, and monitoring of cancer, organ failure …
essential for the identification, early diagnosis, and monitoring of cancer, organ failure …
Multiplex detection of infectious diseases on microfluidic platforms
Infectious diseases contribute significantly to the global disease burden. Sensitive and
accurate screening methods are some of the most effective means of identifying sources of …
accurate screening methods are some of the most effective means of identifying sources of …
Recent advances of food safety detection by nucleic acid isothermal amplification integrated with CRISPR/Cas
J Qiao, Z Zhao, Y Li, M Lu, S Man, S Ye… - Critical Reviews in …, 2024 - Taylor & Francis
Food safety problems have become one of the most important public health issues
worldwide. Therefore, the development of rapid, effective and robust detection is of great …
worldwide. Therefore, the development of rapid, effective and robust detection is of great …