CRISPR/Cas-powered amplification-free detection of nucleic acids: current state of the art, challenges, and futuristic perspectives

Y Li, Y Liu, X Tang, J Qiao, J Kou, S Man, L Zhu… - ACS …, 2023 - ACS Publications
CRISPR/Cas system is becoming an increasingly influential technology that has been
repositioned in nucleic acid detection. A preamplification step is usually required to improve …

CRISPR/Cas and argonaute-based biosensors for pathogen detection

Z Yang, S Mao, L Wang, S Fu, Y Dong… - ACS …, 2023 - ACS Publications
Over the past few decades, pathogens have posed a threat to human security, and rapid
identification of pathogens should be one of the ideal methods to prevent major public …

Argonaute-DNAzyme tandem biosensing for highly sensitive and simultaneous dual-gene detection of methicillin-resistant Staphylococcus aureus

Y Li, X Tang, N Wang, Z Zhao, S Man, L Zhu… - Biosensors and …, 2024 - Elsevier
Methicillin-resistant Staphylococcus aureus (MRSA), a common zoonotic multidrug-resistant
bacterium, puts a great threat to public health and food safety. Rapid and reliable detection …

CRISPR-powered strategies for amplification-free diagnostics of infectious diseases

Y **a, R Rao, M **ong, B He, B Zheng, Y Jia… - Analytical …, 2024 - ACS Publications
Infectious diseases continue to present significant challenges globally, with high mortality
rates and escalating healthcare costs. The Clustered Regularly Interspaced Short …

Next-generation pathogen detection: Exploring the power of nucleic acid amplification-free biosensors

Y Wang, Z Wang, Y Shang, J Wang, Z Zhu, L **… - Coordination Chemistry …, 2024 - Elsevier
Abstract Accurate, rapid, cost-effective, and point-of-care pathogen identification is crucial for
public health safety and encompasses disease prevention, food safety, and environmental …

A portable deep-learning-assisted digital single-particle counting biosensing platform for amplification-free nucleic acid detection using a lens-free holography …

Y Zhou, J Zhao, R Chen, P Lu, W Zhao, R Ma, T **ao… - Nano Today, 2024 - Elsevier
The digital single-particle assay has emerged as a highly promising technology in various
detection applications, such as food safety inspection, environmental monitoring, and in vitro …

One-pot isothermal amplification permits recycled activation of CRISPR/Cas12a for sensing terminal deoxynucleotidyl transferase activity

J Xu, H Yang, Z Sui, X Yuan, L Jia, L Guo - Chemical Communications, 2024 - pubs.rsc.org
This study introduces a one-pot isothermal amplification assay for ultrasensitive analysis of
terminal deoxynucleotidyl transferase (TdT) activity. The system realizes recycled activation …

Ultrasensitive ImmunoMag-CRISPR lateral flow assay for point-of-care testing of urinary biomarkers

I Lee, SJ Kwon, P Heeger, JS Dordick - ACS sensors, 2023 - ACS Publications
Rapid, accurate, and noninvasive detection of biomarkers in saliva, urine, or nasal fluid is
essential for the identification, early diagnosis, and monitoring of cancer, organ failure …

Multiplex detection of infectious diseases on microfluidic platforms

F Chen, Q Hu, H Li, Y **e, L **u, Y Zhang, X Guo, K Yin - Biosensors, 2023 - mdpi.com
Infectious diseases contribute significantly to the global disease burden. Sensitive and
accurate screening methods are some of the most effective means of identifying sources of …

Recent advances of food safety detection by nucleic acid isothermal amplification integrated with CRISPR/Cas

J Qiao, Z Zhao, Y Li, M Lu, S Man, S Ye… - Critical Reviews in …, 2024 - Taylor & Francis
Food safety problems have become one of the most important public health issues
worldwide. Therefore, the development of rapid, effective and robust detection is of great …