A practical guide to DNA metabarcoding for entomological ecologists

M Liu, LJ Clarke, SC Baker, GJ Jordan… - Ecological …, 2020 - Wiley Online Library
1. DNA metabarcoding is a cost‐effective species identification approach with great potential
to assist entomological ecologists. This review presents a practical guide to help …

Beyond biodiversity: Can environmental DNA (eDNA) cut it as a population genetics tool?

CIM Adams, M Knapp, NJ Gemmell, GJ Jeunen… - Genes, 2019 - mdpi.com
Population genetic data underpin many studies of behavioral, ecological, and evolutionary
processes in wild populations and contribute to effective conservation management …

Meta‐analysis shows both congruence and complementarity of DNA and eDNA metabarcoding to traditional methods for biological community assessment

F Keck, RC Blackman, R Bossart… - Molecular …, 2022 - Wiley Online Library
DNA metabarcoding is increasingly used for the assessment of aquatic communities, and
numerous studies have investigated the consistency of this technique with traditional …

Toward quantitative metabarcoding

AO Shelton, ZJ Gold, AJ Jensen, ED′ Agnese… - Ecology, 2023 - Wiley Online Library
Amplicon‐sequence data from environmental DNA (eDNA) and microbiome studies provide
important information for ecology, conservation, management, and health. At present …

Combining ddPCR and environmental DNA to improve detection capabilities of a critically endangered freshwater invertebrate

Q Mauvisseau, J Davy-Bowker, M Bulling, R Brys… - Scientific reports, 2019 - nature.com
Isogenus nubecula is a critically endangered Plecoptera species. Considered extinct in the
UK, I. nubecula was recently rediscovered (in one location of the River Dee, Wales), after 22 …

[HTML][HTML] Zooplankton biodiversity monitoring in polluted freshwater ecosystems: A technical review

W **ong, X Huang, Y Chen, R Fu, X Du, X Chen… - Environmental Science …, 2020 - Elsevier
Freshwater ecosystems harbor a vast diversity of micro-eukaryotes (rotifers, crustaceans and
protists), and such diverse taxonomic groups play important roles in ecosystem functioning …

Environmental DNA gives comparable results to morphology-based indices of macroinvertebrates in a large-scale ecological assessment

J Brantschen, RC Blackman, JC Walser, F Altermatt - PloS one, 2021 - journals.plos.org
Anthropogenic activities are changing the state of ecosystems worldwide, affecting
community composition and often resulting in loss of biodiversity. Rivers are among the most …

Assessment of benthic invertebrate diversity and river ecological status along an urbanized gradient using environmental DNA metabarcoding and a traditional survey …

F Ji, D Han, L Yan, S Yan, J Zha, J Shen - Science of the Total Environment, 2022 - Elsevier
Benthic invertebrate diversity is one of the most commonly used bioindicators for assessing
aquatic ecosystem health in river systems. Although an increasing number of studies have …

Quantification of environmental DNA (eDNA) shedding and decay rates for three commercially harvested fish species and comparison between eDNA detection and …

A Kirtane, D Wieczorek, T Noji, L Baskin… - Environmental …, 2021 - Wiley Online Library
Stock assessments are critical to inform decisions for sustainable fisheries management.
Environmental DNA (eDNA) analysis is a potential tool for assessing fish biomass and …

VESPA: an optimized protocol for accurate metabarcoding-based characterization of vertebrate eukaryotic endosymbiont and parasite assemblages

LA Owens, S Friant, B Martorelli Di Genova… - Nature …, 2024 - nature.com
Protocols for characterizing taxonomic assemblages by deep sequencing of short DNA
barcode regions (metabarcoding) have revolutionized our understanding of microbial …